Here, we present shRNA transgenic mice that via doxycycline (DOX) regulable expression of a highly efficient miR30-E-based shRNA enabled knockdown of Atg7 simultaneously in the majority of organs, with the brain and spleen being noteable exceptions.

Atg7 knockdown enhances chemosensitivity in AML cells. (A) Atg7 knockdown (ShAtg7) or nonsilencing scrambled control (ShControl) OCI-AML3 AML cells were treated with Ara-C (2 μM) or (B) Ida (5 ng/mL) for different time intervals, and …

ATG7 was effectively downregulated in MSC-shRNA 3 cells. shRNA clone 3 was selected as a proper vector for autophagy suppression in hBM-MSCs. Expression of β-actin was also evaluated for normalization.

Dec 11, 2023 · We report that autophagy-related gene 7 (ATG7) modulates p53 activity to regulate cell cycle and survival during metabolic stress, and that indicates Atg7 is functionally involved in cellular...

May 1, 2020 · To specifically knock down ATG7 gene in NHEM, the ATG7 short hairpin RNA (shRNA) and control shRNA were transfected. Quantitative reverse transcriptase in real-time PCR (qRT-PCR) analysis...

Tumorigenesis in livers induced by HRAS G12V and p53 shRNA was significantly suppressed by Atg7 knockdown. The inhibition of autophagy led to a decreased proliferation of cancer cells, as determined by Ki-67 staining.

Sep 1, 2021 · Formation of autophagosome was suppressed in nutrient-starved Hep3B cells expressing Atg7 shRNA, demonstrating that it efficiently inhibited autophagy in HCC cells. Transposons encoding Atg7 shRNA were mixed with those expressing HRAS G12V and p53 shRNA, and subsequently used for hydrodynamic injection to 5-week-old C57BL/6 mice.

Feb 11, 2025 · Acute systemic knockdown of Atg7 is lethal and causes pancreatic destruction in shRNA transgenic mice. MiR-146a-5p accelerates sepsis through dendritic cell activation and glycolysis via targeting ATG7. ATG7 is a haploinsufficient repressor of tumor progression and promoter of metastasis.

Nov 28, 2023 · To further confirm the requirement for ataxia telangiectasia mutated (ATM) in ATG7 ubiquitination, we determined the effect of short hairpin RNA (shRNA)-induced stable knockdown of ATM and inhibition of ATM activity on ATG7 ubiquitination.

May 1, 2020 · To specifically knock down ATG7 gene in NHEM, the ATG7 short hairpin RNA (shRNA) and control shRNA were transfected. Quantitative reverse transcriptase in real-time PCR (qRT-PCR) analysis showed that the mRNA expression of ATG7 in NHEM was significantly decreased in ATG7 shRNA group (Fig. 1a).