Mar 11, 2020 · In addition, chromatin immunoprecipitation sequencing (ChIP-seq) and ChIP quantitative PCR (qPCR) experiments show that NM1 is enriched at the transcription start site (TSS) of the p21 gene and...

The mRNA expression of p21 (upper graph) and p27 (lower graph) was determined by RT-qPCR 24 h after infection. Data are represented relative to the expression in Ad-GFP-infected cell. B. Heat-map showing the 82 genes changed by p21 related to cell cycle and cell division with an expression change > 2.3-fold.

Nov 22, 2023 · Herein, we identify the human RNA binding motif protein 42 (RBM42) as a regulator of p21 levels during DNA damage. Genome-wide transcriptome and interactome analysis reveals that RBM42 alters the...

Here we uncover key age-specific differences underlying this proliferative decline: namely, the genetic loci of CDK inhibitors (CDKI) p21 and p16 are more epigenetically silenced in young muscle stem cells, as compared to old, both in quiescent cells and …

Sep 3, 2010 · We found that PCBP4 expression decreases cyclin-dependent kinase inhibitor p21 induction in response to DNA damage. We also provided evidence that PCBP4 regulates p21 expression independently of p53. In addition, we showed that a deficiency in PCBP4 enhances p21 induction upon DNA damage.

Our study illuminates that immediate-early cell-autonomous reactions to cellular stress are coordinated with cell non-autonomous responses through p21-mediated changes in the Rb-dependent transcriptional landscape to establish a biological timer mechanism for …

Sep 14, 2017 · We observed extra-telomeric binding of the telomere repeat binding factor TRF2 within the promoter of the cyclin-dependent kinase CDKNIA (p21/CIP1/WAF1). This result in TRF2 induced...

Sep 17, 2016 · The expression levels of p21 were evaluated 72 h or 96 h later using quantitative real-time PCR (RT-qPCR) and immunoblotting. As shown in Fig. 1b and c , dsP21-322 significantly increased p21 mRNA and protein levels compared with mock and dsControl transfections in the aforementioned urologic tumor cells.

Jun 5, 2014 · LincRNA-p21 acts with hnRNP-K as a transcriptional co-activator of p53-mediated expression of p21 (A) Schematic of the positions of qPCR primers used for ChIP analysis, indicating the distance (base pairs) relative to the p21 transcription start site (TSS).

A ready-to-use mix for SYBR Green qPCR, containing ROX reference and suitable for all qPCR instruments without adjusting the concentration of ROX. 4 x 1.25 ml (500 rxns/20ul reaction)