May 31, 2007 · Here, we describe a protocol for cDNA-AFLP, an AFLP-based transcript profiling method that allows genome-wide expression analysis in any species without the need for prior sequence...

Amplified fragment length polymorphism (AFLP) is a PCR-based technique that uses selective amplification of a subset of digested DNA fragments to generate and compare unique fingerprints for genomes of interest.

Amplified fragment length polymorphism (AFLP-PCR or AFLP) is a PCR-based tool used in genetics research, DNA fingerprinting, and in the practice of genetic engineering.

In essence, the cDNA-AFLP method involves reverse transcription of mRNA into double-stranded cDNA, followed by restriction digestion, ligation of specific adapters and fractionation of this mixture of cDNA fragments into smaller subsets by selective PCR amplification.

cDNA/AFLP Protocol Preparation of Para-magnetic beads from Promega cat#Z5482 : a) suspend magnetic particles in bottle - transfer 200 ul (200 ug) of beads per sample

Jun 30, 2009 · Results confirmed the altered expression patterns of 28 (96.5%) genes revealed by the cDNA-AFLP technique. The results show that cDNA-AFLP is a reliable technique for studying expression patterns of genes involved in the wheat-stripe rust interactions.

May 23, 2024 · The cDNA-AFLP method involves the reverse transcription of mRNA into double-stranded cDNA, followed by restriction digestion, ligation of specific adapters, and fractionation of the resulting cDNA fragments into smaller subsets through selective PCR amplification.

Oct 13, 2008 · With the goal of improving the coverage of cDNA-AFLP without increasing its redundancy, we designed a modified cDNA-AFLP protocol. Immobilized cDNA is sequentially digested with several restriction endonucleases and the released DNA fragments are collected in mutually exclusive pools.

Mar 18, 2003 · An improved cDNA-AFLP method for genome-wide expression analysis has been developed. We demonstrate that this method is an efficient tool for quantitative transcript profiling and a valid alternative to microarrays.

The cDNA-AFLP technique is especially useful for profiling of transcriptional responses of jasmonate-treated plants or plant (tissue) cultures and the discovery of jasmonate-responsive genes.