In this review, we describe the development of reagents and methods used for BiFC-based screens in yeast, plants, and mammalian cells. We also discuss the advantages and drawbacks of these methods and highlight the application of BiFC in large-scale studies.

The bimolecular fluorescence complementation (BiFC) assay, also referred to as “split-fluorescent protein” technology (e.g., split-YFP), is one of the most popular and frequently used methods in the plant field to study protein-protein interactions in vivo (reviewed in [3]).

Jun 27, 2019 · Because of their ability to provide spatial and temporal information, imaging tools based on Föster Resonance Energy Transfer (FRET) 1 or bimolecular fluorescence complementation (BiFC) 2...

May 30, 2014 · Since its introduction in plants 10 years ago, the bimolecular fluorescence complementation (BiFC) method, or split-YFP (yellow fluorescent protein), has gained popularity within the plant biology field as a method to study protein-protein interactions.

Bimolecular fluorescence complementation (also known as BiFC) is a technology typically used to validate protein interactions. It is based on the association of fluorescent protein fragments that are attached to components of the same macromolecular complex.

Bimolecular fluorescence complementation, or BiFC, is a technique capable of detecting these protein–protein events essential for 7TM receptor function. Fluorescent proteins, such as those from Aequorea victoria, are split into two non-fluorescent …

Sep 28, 2021 · In this study, we report the development of a screening approach which utilizes BiFC and applies flow cytometry to characterize membrane protein interaction partners in the host Saccharomyces...

Jul 15, 2023 · In this chapter, we describe the widely used split yellow fluorescent protein (YFP) system to visualize the protein–protein interaction in plant cells. Bimolecular fluorescence complementation (BiFC) is an important technique to verify protein–protein interaction in vivo in the most native system.

Bi-molecular fluorescence complementation (BiFc) is a fluorescent protein biosensor technique where two halves of a fluorescent protein (e.g. YFP) are fused to two different proteins [65,66].

Sep 26, 2016 · Our approach utilizes bimolecular fluorescence complementation (BiFC), whereby each protein of an interaction pair is fused to nonfluorescent fragments of yellow fluorescent protein (YFP) that combine and mature as the complex is formed.