The enzyme Uridine monophosphate synthase (EC 4.1.1.23, UMPS) (orotate phosphoribosyl transferase and orotidine-5'-decarboxylase) catalyses the formation of uridine monophosphate (UMP), an energy-carrying molecule in many important biosynthetic pathways. [5]

A recent study has shown that resting/low proliferating cells rely mainly on the nucleotide salvage pathway to synthesize RNA [16]. Also, uridine can be obtained from UTP and cytosine 5′-triphosphate (CTP) via the pyrimidine salvage pathway.

Uridine monophosphate (UMP), also known as 5′-uridylic acid (conjugate base uridylate), is a nucleotide that is used as a monomer in RNA. It is an ester of phosphoric acid with the nucleoside uridine.

UMP is converted to uridine-5′-triphosphate (UTP) by two sequential reactions with ATP involving two kinases (UMP and UDP kinases). UTP is aminated, by a reaction catalyzed by a CTP synthase, using ammonia derived from l -glutamine as source of nitrogen, to yield CTP.

Jan 1, 1996 · The de novo pathway forms orotate from the normally available precursors aspartate, glutamine, and bicarbonate, and UMP synthase then converts orotate to UMP. UMP synthase contains two separate catalytic domains for the two sequential reactions, orotate phosphoribosyltransferase (OPRTase) and orotidylate decarboxylase (ODCase) shown in Fig. 1.

Jan 3, 2020 · The de novo pyrimidine biosynthetic pathway, the synthesis of uridine-5'-monophosphate (UMP) from carbamoyl-phosphate, is evolutionarily conserved from prokaryotes to eukaryotes. The pathway consists of the synthesis of carbamoyl-aspartate, formation of orotate, and the production of UMP.

Biosynthesis of UMP takes place in six steps and is catalyzed by three enzymes in humans. The first three transformations that put together carbonate, ATP, the nitrogen from glutamine, and aspartate to yield dihydroorotate, are catalyzed by the enzyme CAD.

Jan 17, 2019 · About This Pathway The de novo pyrimidine nucleotide biosynthetic pathway converts HCO3, GLN, L-ASPARTATE and PRPP (PRPP) to UMP (uridine 5'-monophosphate), a pyrimidine nucleotide that can be subsequently converted to …

UMPS is a bifunctional two-domain enzyme that catalyzes the final two steps of the de novo pyrimidine biosynthesis pathway to generate UMP (Jones, 1980). In the first step, the orotate phosphoribosyltransferase (OPRT) domain synthesizes OMP from orotate and PRPP, and in the second, the OMP decarboxylase (ODC) domain converts OMP to UMP (Figure 6A).

Mar 2, 2011 · Uridine-5′-monophosphate synthase (UMPS), the critical step of the de novo pyrimidine biosynthesis pathway, which is a housekeeping plastid process in higher plants, was investigated in a marine diatom, the most crucial primary producer in the marine environment.