Here, we show that all seven UBX domain proteins of Saccharomyces cerevisiae bind to Cdc48, thus constituting an entire new family of Cdc48 cofactors. The two major yeast UBX domain …

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人类基因组中的四个基因编码泛素： UBB， UBC， UBA52 和 RPS27A。 泛素具有7个赖氨酸残基（K6，K11，K27，K29，K33，K48，K63）和一个甲硫氨酸残基（M1）。 泛素之间主要通 …

Shown are the known Ub ligases (left) mediating MYC ubiquitination, DUBs (upper right) for MYC deubiquitination and SENP1 (lower right) that deSUMOylates and stabilizes MYC.

Sep 1, 2004 · (B) Anti-myc IP of ubiquitylated proteins from cells expressing myc–ubiquitin (+Ubmyc), followed by WB against myc and HA epitopes. Lysates from strains not expressing …

Dec 15, 2023 · Ubiquitination modification is an important post-translational modification that regulates the stability and function of proteins. Here, we present a protocol to detect the K27 …

His-Ub可能是用来标记Ubiquitin的，Ubiquitin是一个重要的信号分子，它可以和目的蛋白发生共价结合，形成泛素化修饰。 His可能是Histidine的缩写，是一种常用的蛋白质标签。 Flag …

Dec 14, 2009 · Ubiquitination was observed in myc-IPs using anti-Ub, and loading was determined by using anti-myc antibody. The anti-myc WB was overexposed to show the anti …

The canonical targeting signal for degrading proteins by the ubiquitin (Ub) system—a chain composed of multiple Ub moieties—has remained a mystery. The structure of the proteasome, …

Aug 1, 2004 · Here, we show that all seven UBX domain proteins of Saccharomyces cerevisiae bind to Cdc48, thus constituting an entire new family of Cdc48 cofactors. The two major yeast …