Mammalian Atg8 proteins and the autophagy factor IRGM control …
Aug 3, 2020 · Here, we show that mammalian Atg8 proteins (mAtg8s) and the autophagy regulator IRGM control TFEB, a transcriptional activator of the lysosomal system. IRGM …
MCOLN1 is a ROS sensor in lysosomes that regulates autophagy
Jun 30, 2016 · Here, the authors show that ROS can induce autophagy by triggering TRPML1 to release Ca2+from the lysosomal lumen, in turn activating the autophagy and lysosomal …
Erbin accelerates TFEB-mediated lysosome biogenesis and …
Dec 17, 2023 · To explore how Erbin regulates lysosomal biogenesis, we investigated whether Erbin associates with TFEB. We transferred several plasmids in 293T cells, we found that …
Lactylation stabilizes TFEB to elevate autophagy and lysosomal …
We found that lactate-induced lactylation of TFEB at the lysine 91 (K91) site protects TFEB from E3 ubiquitin ligase WWP2-mediated ubiquitination and proteasomal degradation, leading to …
TFEB regulates pluripotency transcriptional network in mouse …
However, in the present study, we propose a novel mechanism for TFEB governing pluripotency of mouse ESCs (mESCs) by regulating the pluripotency transcriptional network (PTN) in these …
The C-ETS2-TFEB Axis Promotes Neuron Survival under Oxidative …
To determine whether the upregulation of TFEB occurred at the transcriptional level, we transfected a PGL3 construct containing the TFEB promoter into 293T cells and measured …
TFEB overexpression in HEK-293 APPswe or C99 expressing
TFEB overexpression in HEK-293 APPswe or C99 expressing cells leads to the induction of numerous lysosomal-autophagic genes and to reduced C99 levels. (A) Bars show the …
A TBK1-independent primordial function of STING in ... - Cell Press
Sep 19, 2024 · TFEB/TFE3 activation via the channel of STING integrates cytosolic DNA sensing, a general cellular stress-sensing mechanism, with lysosomal biogenesis and autophagy …
Lactate upregulates TFEB protein levels. (A) TFEB levels in HeLa ...
The transcription factor TFEB is a major regulator of lysosomal biogenesis and autophagy. There is growing evidence that posttranslational modifications play a crucial role in regulating TFEB...
TFEB SUMOylation in macrophages accelerates atherosclerosis by ...
Nov 11, 2023 · We overexpressed wild-type and mutant Flag-TFEB in 293T cells, and then enriched TFEB protein by immunoprecipitation (Fig. 6A). Through mass spectrometry analysis, …