We show that a recombination-based cloning protocol facilitates simple and rapid transfer of the TcR transgene into different expression systems. The presented comprehensive method can be performed in any laboratory with standard equipment and with a …

Dec 6, 2022 · T cells are able to detect and respond to specific peptide antigens by virtue of the unique T cell receptor (TCR) complexes they express on their cell surface (see image).

Apr 7, 2022 · We used the detection-and-cloning system to clone a tumour-antigen-specific TCR from peripheral blood mononuclear cells of healthy donors. TCR cloning by leveraging the cis activation of T...

We also present a TCR cloning strategy to specifically amplify TCRs from T cell populations. Positioned downstream of single-cell or bulk TCR sequencing, it allows time- and cost-effective discovery, cloning, screening, and engineering of tumor-specific TCRs.

Jul 6, 2022 · In a recent article published in Nature Biomedical Engineering, Kobayashi and colleagues revealed a novel T cell activation induced by cis interaction, and developed the T-cell immunospot array...

In this review, we introduce two novel approaches for immunological interpretation of the TCR repertoire. Inter-organ clone tracking analysis based on bulk TCRseq can enrich tumor-reactive T-cell clones that are mobilized into the cancer-immunity cycle, providing a more direct index of the antitumor T-cell response than repertoire diversity or ...

2 days ago · Figure 1. Characterizes single Treg TCR and dual TCR Treg in the Blood, Kldney, Liver, LPL, and Pancreas of mice. A, Mouse source names, GEO accession number, total number of paired TCR Treg cells, and number and proportion of TCR paired types.B, Four different TCR pairing types, VDJ gene family names and CDR3 AA sequences in single T cells.C, Proportion of TCR pairing types per mouse.

Dec 30, 2024 · Implementing newly created DALI software for TCR repertoire analysis in single-cell analysis enabled the rapid selection of the ideal responder CD8 T cell clone, based on antigen reactivity, proliferation, and immunophenotype in vivo.

Mar 19, 2025 · A TCR-seq analysis revealed the presence of full-length TCR sequences in approximately half of the T cells (Fig. 3D; ... Analysis of abundant T cell clones demonstrated that the phenotype of each clone was dominated by CD8 T EM or T CM cells, with scattered cells of different subset phenotypes (e.g., CD8 T PEX) (fig. S8B).

We also present a TCR cloning strategy to spe-cifically amplify TCRs from T cell populations. Positioned downstream of single-cell or bulk TCR sequencing, it allows time- and cost-effective discovery, cloning, screening, and engineering of tumor-specific TCRs.