The 293T cell line, originally referred as 293tsA1609neo, is a highly transfectable derivative of human embryonic kidney 293 cells, and contains the SV40 T-antigen. This cell line is competent to replicate vectors carrying the SV40 region of replication. It gives high titers when used to produce retroviruses.

Human embryonic kidney 293 cells, also often referred to as HEK 293, HEK-293, 293 cells, are an immortalised cell line derived from HEK cells isolated from a female fetus in the 1970s. [1] [2] The HEK 293 cell line has been widely used in research for decades due to its reliable and fast growth and propensity for transfection.

293 [HEK-293] is a cell line exhibiting epithelial morphology that was isolated from the kidney of a human embryo. This cell line can be used in industrial biotechnology and toxicology research. It has applications in efficacy testing and viruscide testing.

Here, we provide a step-by-step protocol for cell culture, plasmid transfection in HEK293T, and adenoviral infection in C2C12 cells for gene overexpression in vitro, using MG53 as an example. This protocol enables sufficient and efficient gene expression for …

The human cell line, HEK293, have emerged to be the main production platform for therapeutic viral vectors. Advancements in cell line developments would see more HEK293 clones or derivatives developed for growth in a serum-free suspension …

293T is a human cell line that has unique properties useful in many applications, including those requiring efficient transfection, gene expression, DNA replication, or viral production. The cell line is competent for replication of vectors carrying the SV40 origin of replication.

Sep 15, 2015 · However, 293 cells demonstrate no evident tissue-specific gene expression signature and express the markers of renal progenitor cells, neuronal cells and adrenal gland. This complicates efforts to reveal the authentic cell type/tissue of origin.

293T cells that transfect easily and support high-level expression of lentiviral proteins. The Lenti-X 293T Cell Line was clonally selected to meet these requirements, allowing you to produce the highest possible lentiviral titers.

Jun 18, 2021 · Human Embryonic Kidney 293 (HEK 293) cells are one of the most frequently used cell lines for biotechnology and research studies. These cells are quite popular because of their distinct properties like fast-growing, robustness, and ease of maintenance and transfection.

293T is a human cell line that has unique properties useful in many applications, including those requiring efficient transfection, gene expression, DNA replication, or viral production.