Jan 1, 2017 · Using chimeric mice, we show that DC-derived IL-2 is required for maximal Ag-specific proliferation of CD4 + T cells and optimal humoral responses following alum-adjuvanted immunization.

Moreover, SYK couples PAMP recognition to de novo gene transcription and induces chemokine and cytokine production through several downstream pathways including the ERK signalling cascade and activation of transcription factors such as NFAT 94 and NF-κB 11,43,93,95-97.

Activated Syk, an essential tyrosine kinase in B cell signaling, interacts with Vav guanine nucleotide exchange factors and regulates Vav activity through tyrosine phosphorylation. The Vav SH2 domain binds Syk linker B by an unusual recognition of two closely spaced Syk tyrosines: Y342 and Y346.

Spleen tyrosine kinase (Syk) is a nonreceptor tyrosine kinase with a molecular weight of 72 kDa. Syk binds with TLR4 and is activated by phosphorylation mainly at tyrosine residues, resulting in the transduction of stimulatory signals through the …

Aug 16, 2012 · Microbial ligation of pattern-recognition receptors, such as TLR4, CD14, and dectin 1, is now known to induce the activation of calcineurin/NFAT signaling in myeloid cells, a finding that has provided new insights into the molecular pathways that regulate host protection.

In the sole presence of SYK, pathological BCR-signaling in autoreactive or premalignant cells induces NFAT-activation and NFAT-dependent anergy and negative selection.

Jun 25, 2021 · We report that MSCs phagocytose the fungal component zymosan, resulting in phosphorylation of spleen tyrosine kinase (Syk), increase in cytosolic calcium levels, and ultimately, increase in NFAT1 nuclear translocation.

Apr 19, 2021 · Coexpression of ZAP70 with SYK dramatically reduced BCR-induced NFAT nuclear localization (Figures 3E and 3F), whereas total NFAT levels were not altered (Figure S9C).

Jan 1, 2017 · Taken together, our findings identify the Syk/Src–Ca 2+ –NFAT pathway as the master regulator of IL-2 production in DCs upon exposure to sterile particulates. DC-derived IL-2 is necessary for optimal induction of Ag-specific CD4 …

May 14, 2010 · Spleen tyrosine kinase (SYK) is known to have a crucial role in adaptive immune receptor signalling. However, recent reports indicate that SYK also mediates other, unexpectedly diverse...