A stem-loop occurs when two regions of the same nucleic acid strand, usually complementary in nucleotide sequence, base-pair to form a double helix that ends in a loop of unpaired nucleotides. Stem-loops are most commonly found in RNA, and are a key building block of many RNA secondary structures.

May 15, 2024 · What is a stem-loop (hairpin loop)? A Stem-loop can be defined as a hairpin-like pattern formed because of the intramolecular base pairing of the nucleotide sequence (especially in palindromic sequence) within the same molecule.

Mar 17, 2025 · Explore the formation, stability, and functional significance of stem-loop structures in nucleic acids, including their role in gene regulation and molecular processes. Certain nucleic acid sequences fold back on themselves, forming a distinctive stem-loop structure.

Aug 28, 2012 · Hairpin or stem-loop is a commonly observed secondary structure of RNA where A, U, G, C nucleotides form hydrogen bonds with complementary nucleotides on a wrinkled or folded back segment of the same strand (Svoboda & Cara, 2006;

In genetics, a kissing stem-loop, or kissing stem loop interaction, is formed in ribonucleic acid (RNA) when two bases between two hairpin loops pair. These intra- and intermolecular kissing interactions are important in forming the tertiary or quaternary structure of many RNAs.

The RT-primer contains a highly stable stem-loop structure that lengthens the target cDNA. The forward PCR primer adds additional length with nucleotides that optimize its melting temperature (Tm) and enhance assay specificity.

Here we present a systematic analysis of SLS distribution in prokaryotic genomes. Sequences able to fold into stem-loop structures featuring relatively large (12 or more bp) stems have been searched and analyzed in 40 wholly-sequenced bacterial chromosomes.

RNA sequence analysis shows that base stacking and base pairing stabilize the stem–loops that kinetically trap the long-lived intermediates observed in the FDC. Stem–loops formation appears as a general mechanism to explain a wide range of behaviors observed in RNA folding.

MS2 tagging is a technique based upon the natural interaction of the MS2 bacteriophage coat protein with a stem-loop structure from the phage genome, [1] which is used for biochemical purification of RNA-protein complexes and partnered to GFP for detection of …

May 15, 2023 · Stem-loops or hairpin loops are essential structural elements found in nucleic acids, specifically in RNA molecules. They are formed by base pairing between complementary nucleotides within the same strand, leading to the formation of …