Stabilized, feeder-free medium for single-cell passaging of human pluripotent stem cells. Learn More

Learn how to safely freeze human primary cells in ready-to-use, cGMP-manufactured CryoStor® CS10 for long-term storage.

Learn how to generate natural killer cells from pluripotent stem cells using STEMdiff™ and StemSpan™ media and supplements.

Choose ready-to-use fresh or cryopreserved cells, including mononuclear cells (MNCs), purified immune cells, or stem cells isolated from blood or bone marrow, and begin experiments when you are ready.*

Stabilized, feeder-free medium for single-cell passaging of human pluripotent stem cells

Primary human CD34+ cells were isolated from bone marrow (BM) mononuclear cells (MNCs) using positive immunomagnetic separation techniques. CD34 is expressed on hematopoietic stem and progenitor cells.

The colony-forming unit (CFU) assay is one of the most widely used assays for hematopoietic stem and progenitor cells (HSPCs). CFU assays allow measurement of the proliferation and differentiation ability of individual cells within a sample.

STEMCELL offers a wide selection of optimized freezing media that meets your cryopreservation needs for different types of stem cells. The portfolio includes the cGMP-manufactured, serum- and animal component-free CryoStor® and BloodStor® lines of products as well as chemically-defined, serum-free freezing media, such as mFreSR™.

When thawing frozen cells, proper technique and handling ensures optimal viability, recovery, and functionality of the cells for downstream applications. This protocol describes how to thaw frozen primary cells.

Start experiments confidently with ready-to-use, ethically sourced, CD34+ stem and progenitor cells from adult human bone marrow. With personalized service, custom products, flexible delivery times, and the option to reserve entire lots to prescreen cells …