Jul 1, 2023 · To investigate the feasibility of the ratiometric SERS method (I Cy3 to I Rox) for DNA glycosylase assay, we measured the variation of SERS spectra for DNA probes fore-and-aft glycosylase hydrolysis in self-prepared arrayed microreactors.

Jan 15, 2025 · In this study, we designed a novel SERS-based sensing model using a nanoprobe with dual signals for the detection of lung cancer-associated circRNAs (circSATB2) (Zhang et al., 2020). One of the Raman signals originated from the probe molecule (ROX) that was fabricated outside the nanoparticle.

In this work, a ratiometric SERS aptasensor using 6-Carboxyl-X-Rhodamine (ROX)-labeled aptamers and 4-mercaptobenzonitrile (4-MBN)-functionalized gold nanoparticles (Au NPs) as SERS probes was established for the reliable and rapid …

Sep 8, 2017 · Herein, we develop the ratiometric silicon SERS aptasensor for ATP detection, which is made of uniform silver nanoparticles (Ag NPs)-modified silicon wafer (Ag NPs@Si), followed by the functionalization with double-stranded DNA (dsDNA I).

May 15, 2021 · Aptamers, being the unique single stranded DNA/RNA sequences that can specifically recognize and seize the target analytes, combined with Surface-enhanced Raman spectroscopy (SERS), can offer potent multiplex detection capacity with …

Oct 16, 2020 · In this system, DNA-intercalating dye (EVAGreen) and reference dye (ROX) are used together to improve the repeatability and reliability of the SERS signals.

Mar 7, 2017 · A novel aptasensor platform has been developed for quantitative detection of adenosine triphosphate (ATP) based on a ratiometric surface-enhanced Raman scattering (SERS) strategy. The thiolated 3'-Rox-labeled complementary DNA (cDNA) is first immobilized on the gold nanoparticle (AuNP) surface and t …

Jan 5, 2023 · Here, an aptamer-based SERS method for the detection of Staphylococcus aureus (S. aureus) without immobilization using Au@Ag NPs/slide as enhanced substrate was constructed. ROX-aptamer of S. aureus was modified on the surface of Au@Ag NPs/slide through electrostatic interaction.

Mar 15, 2024 · Herein, we developed a dual-signal surface-enhanced Raman scattering (SERS) biosensor for the quantitative detection of miRNA 21 (miR-21) by utilizing a tightly ordered spindle-shaped gold (SAu) array with 5, 5′-dithio-bis- (2-nitrobenzoic acid) (DTNB) molecule as an internal standard.

In this work, a ratiometric SERS aptasensor using 6-Carboxyl-X-Rhodamine (ROX)-labeled aptamers and 4-mercaptobenzonitrile (4-MBN)-functionalized gold nanoparticles (Au NPs) as SERS probes was established for the reliable and rapid …