Despite recent advances in the structural elucidation of SARS-CoV-2 proteins, the detailed architecture of the intact virus remains to be unveiled. Here we report the molecular assembly of the authentic SARS-CoV-2 virus using cryoelectron tomography (cryo-ET) and subtomogram averaging (STA).

Oct 5, 2021 · We define the roles of furin-like proteases, transmembrane protease, serine 2 (TMPRSS2) and cathepsin L in these processes, and delineate the features of ACE2 orthologues in reservoir animal...

Since the first reports of a novel severe acute respiratory syndrome (SARS)-like coronavirus in December 2019 in Wuhan, China, there has been intense interest in understanding how severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) emerged in the human population.

Dec 17, 2024 · In this review, we present a portrait of selected cellular pathways perturbed in SARS-CoV-2 infection: the effect of viral translation, replication and assembly on the morphology and function of...

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), also known as “the novel coronavirus” due to genome variation relative to previously identified coronaviruses, is a positive sense RNA virus and the etiological agent of COVID-19.

Especially, mitochondria and cell membrane are crucial for the course of infection. The SARS-CoV-2 invasion depends on angiotensin converting enzyme 2 (ACE2), transmembrane serine protease 2 (TMPRSS2), and cluster of differentiation 147 (CD147), expressed on host cells.

May 19, 2022 · Understanding the structure and function of SARS-CoV-2 and its variants is essential in developing vaccines and therapies to tackle the COVID-19 disease. Updated May 19, 2022. Overview. Early genomic analysis of SARS-CoV-2 revealed that it belongs to the betacoronavirus genus, lineage B, alongside SARS-CoV-2 1, 2.

Mar 31, 2025 · Author summary As SARS-CoV-2 continues to spread and adapt in humans, viral variants with enhanced spread and immune evasion have emerged throughout the COVID-19 pandemic. While most of the mutations occur in the Spike protein and have been extensively studied, non-Spike mutations have been accumulating and are not as well understood. Here, …

Mar 14, 2025 · Severe acute respiratory syndrome coronavirus (SARS-CoV) (1–4) and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) (5–9) have crossed the species barrier to infect humans.Both SARS-CoV and SARS-CoV-2 have been identified to use their spike (S) proteins on the virus surface to bind human angiotensin-converting enzyme 2 (hACE2) as the …

The present study provides evidence that host cell entry of SARS-CoV-2 depends on the SARS-CoV receptor ACE2 and can be blocked by a clinically proven inhibitor of the cellular serine protease TMPRSS2, which is employed by SARS-CoV-2 for S protein priming.