Nuclease S7 is an endonuclease that mainly cleaves single-stranded substrates. It also cleaves double-stranded DNA or RNA. [5] It cleaves the 5′-phosphodiester bonds of RNA and DNA to yield 3′-mononucleotides and oligonucleotides.

CUT&RUN sequencing, antibody-targeted controlled cleavage by micrococcal nuclease for transcriptomic profiling. Hydrolysis of nucleic acids in crude cell-free extracts. Sequencing of RNA. Preparation of rabbit reticulocyte lysates. Studies of chromatin structure.

Micrococcal nuclease is derived from Staphylococcus aureus and is a relatively non-specific endo-exonuclease. It is purified from a recombinant E. coli strain that digests double-stranded, single-stranded, circular and linear nucleic acids.

Deoxyribonuclease (DNase, for short) refers to a group of glycoprotein endonucleases which are enzymes that catalyze the hydrolytic cleavage of phosphodiester linkages in the DNA backbone, thus degrading DNA.

Micrococcal Nuclease (S7 Nuclease) is a relatively nonspecific endo-exonuclease that digests single-stranded and double-stranded nucleic acids, but is more active on single-stranded substrates. Cleavage of DNA or RNA occurs preferentially at AT or AU-rich regions, yielding mononucleotides and oligonucleotides with terminal 3'-phosphates.

Sep 15, 2016 · As discussed above, RNase I and A are a bad choice for ribosome profiling in mice due to ribosomal degradation (Figure 1), hence there are only two alternatives: micrococcal nuclease S7 and nuclease T1. We sequenced libraries from liver lysates treated with either S7, T1 or a combination of nucleases.

Aug 2, 2024 · Deoxyribonuclease 2 (DNase II) plays a key role in clearing cytoplasmic double-stranded DNA (dsDNA). Deficiency of DNase II leads to DNA accumulation in the cytoplasm. Persistent dsDNA in neurons is an early pathological hallmark of senescence and neurodegenerative diseases including Alzheimer’s disease (AD).

We show here that, in the presence of Mn 2+ and low-level inorganic phosphate (P i), PNPase degrades ssDNA. The limited end-processing of DNA is regulated by ATP and is inactive in the presence of Mg 2+ or high-level P i.

提供了用于DNase I失活所需的EDTA。 用途：制备不含DNA的RNA样品；RT-PCR反应前RNA样品中去除基因组DNA等可能的DNA污染；体外T7, T3, SP6等RNA Polymerases催化的RNA转录后去除DNA模板； DNase I footprinting研究DNA-蛋白质相互作用；缺口平移(nick

Nuclease S7 is an endonuclease that mainly cleaves single-stranded substrates. It also cleaves double-stranded DNA or RNA. It cleaves the 5′-phosphodiester bonds of RNA and DNA to yield 3′-mononucleotides and oligonucleotides.