Tetracycline-controlled transcriptional activation is a method of inducible gene expression where transcription is reversibly turned on or off in the presence of the antibiotic tetracycline or one of …

Plasmid pLenti CMV rtTA3 Blast (w756-1) from Dr. Eric Campeau's lab contains the insert Tetracycline repressor A3 mutant. This plasmid is available through Addgene.

When compared with rtTA2 S-S2, the new rtTA S12G+F86Y+A209T variant (a.k.a. rtTA3 ) was 5-fold more active at high dox levels and required 25-fold less dox to be similarly active (25-fold …

突变这些残基导致了一种反Tet阻遏物 (rTetR)的产生，它逆转了表型，并依赖四环素的存在进行诱导，而不是抑制。 通过将rTetR与VP16融合，产生了新的转激活子 rtTA (反向四环素控制转激 …

Here we describe the generation of two mouse strains that enable Cre-dependent, robust expression of rtTA3, providing tissue-restricted and consistent induction of TRE -controlled …

These mice are a Tet-On tool strain - allowing dox-conditional expression of TRE promoter-driven target genes.

I am going to construct my own cassette which will be Dox inducible, i came across several names of tetracycline activator (rtTA). upon sequence alignment i found some differences. For …

Jun 10, 2019 · To replace TetON3G, the fragment of reverse tetracycline-transactivator 3 (rtTA3) PCR-amplified from plasmid pTRIPZ was digested with BstBI and SmaI, and ligated to equally …

Apr 17, 2014 · Here we describe the generation of two mouse strains that enable Cre-dependent, robust expression of rtTA3, providing tissue-restricted and consistent induction of TRE …

This CAGs-rtTA3 transgenic strain is a component of a novel system applying conditional RNA interference (RNAi) to knock out targeted genes.</p>