Tetracycline-controlled transcriptional activation is a method of inducible gene expression where transcription is reversibly turned on or off in the presence of the antibiotic tetracycline or one of its derivatives (e.g. doxycycline). [1]

The new transactivator rtTA ( reverse tetracycline-controlled transactivator) was created by fusing rTetR with VP16. The tetracycline on system is also known as the rtTA-dependent system.

Apr 1, 2015 · In the Tet-Off expression system, a tetracycline-controlled transactivator protein (tTA), which is composed of the Tet repressor DNA binding protein (TetR) from the Tc resistance operon of Escherichia coli transposon Tn10 fused to the strong transactivating domain of VP16 from Herpes simplex virus, regulates expression of a target gene that is u...

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We here review how a dox-controlled HIV-1 variant was designed and used to greatly improve the activity and dox-sensitivity of the rtTA transcriptional activator component of the Tet-On system.

The ROSA-rtTA-M2 Tet-On rat line may provide a versatile tool for functional genetic studies because rtTA is ubiquitously expressed and can regulate conditional expression of target transgenes, in vitro or in vivo.

Jun 21, 2016 · The reverse tetracycline transactivator (rtTA) combines a variant of the strong DNA binding domain from the TetR regulator of the bacterial tetracycline resistance transposon with a highly...

Apr 1, 2002 · The reverse tetracycline-controlled transcriptional activator (rtTA) activates the responsive elements only in the presence of doxycycline, giving a convenient control over the target transgene. The recently developed tetracycline-controlled transcriptional silencer (tTS) has been successfully used in cultured cells and in transgenic mice.

Oct 2, 2019 · The reverse-tTA (rtTA) is a tTA variant allowing gene expression activation in presence of an inducer; the resulting Tet-On system is generally preferred when rapid and dynamic gene induction...

The reverse tetracycline-controlled transcriptional activator (rtTA) activates the responsive elements only in the presence of doxycycline, giving a convenient control over the target transgene. The recently developed tetracycline-controlled transcriptional silencer (tTS) has been successfully used in cultured cells and in transgenic mice.