Plasmid EGFP-Rab7A T22N from Dr. Qing Zhong's lab contains the insert Rab7A and is published in Proc Natl Acad Sci U S A. 2010 Nov 9. 107(45):19338-43. This plasmid is available through Addgene.

Oct 1, 2003 · The ability of Rab7 T22N expression and reduced Rab7 protein levels to protect from growth factor withdrawal-induced apoptosis strongly suggests that the Rab7-dependent degradation of nutrient transporters contributes to the initiation of growth factor withdrawal-induced apoptosis.

Oct 13, 2017 · In this study, we have expressed Enhanced Green Fluorescent Protein (EGFP)–tagged Rab7 wt as well as active and dominant-negative mutant proteins and analyzed their effect on the localization and other properties of late endocytic structures by confocal and electron microscopy.

Jan 17, 2014 · In cells expressing dominant-negative Rab7 (T22N), the GFP-LC3 signal slightly accumulated around GAS and no GAS was enclosed in GcAV-like membrane-delimited structures.

Two mutants of RAB7 are widely used to study the function of this GTPase: a dominant negative mutant (RAB7 T22N) characterized by impaired nucleotide exchange, thus locking the protein in its inactive GDP-bound form, and a constitutively active mutant (RAB7 Q67L) characterized by impaired GTP hydrolysis, thus locking the protein in its active ...

We have now used a new approach to analyze the role of Rab7: transient expression of Enhanced Green Fluorescent Protein (EGFP)–tagged Rab7 wt and mutant proteins in HeLa cells. EGFP-Rab7 wt was associated with late endocytic structures, mainly lysosomes, which aggregated and fused in the perinuclear region.

Nov 3, 2020 · We find that active Rab7 interacts with the NPC1 cholesterol transporter and licenses lysosomal cholesterol export. This process is abolished in C18orf8 -, Ccz1 - and Mon1A/B -deficient cells and...

Nov 20, 2017 · RAB7a KO cells transduced with the indicated GFP‐RAB7a variants were fixed and co‐stained for endogenous LAMP2. Note that the inactive RAB7 (T22N) does not localize to lysosomes, whereas the hyperactive variant (Q67L) fully localizes to lysosomes.

Our data demonstrate that injected Rab7 WT stimulates HRP uptake. Rab7:T22N, the dominant-negative mutant of Rab7 (Feig et al., 1988; Nuoffer et al., 1994; Riederer et al., 1994), was found to be mildly inhibitory for HRP uptake when injected alone but was fully able to block the stimulatory effect of Rab7. As outlined above for inhibition by ...

Jun 1, 2004 · Rab7 is a member of the Rab family involved in transport to late endosomes and in the biogenesis of the perinuclear lysosome compartment. To assess the role of Rab7 in autophagy we stably transfected CHO cells with wild-type pEGFP-Rab7, and the mutants T22N (GDP form) and Q67L (GTP form).