Aug 16, 1999 · We demonstrate that the SCV recruits pre-existing lysosomal glycoproteins (Lgps) in a rab7-dependent manner, without directly interacting with lysosomes. We also show the transient accumulation, in the vicinity of the SCV, of novel rab7- and Lgp-containing vesicles containing very low amounts of cathepsin D.

We found that the initial centripetal displacement of the SCV is due to recruitment by Rab7 of Rab7-interacting lysosomal protein (RILP), an effector protein that can simultaneously associate with the dynein motor complex.

Aug 16, 1999 · We show that rab7 is present on SCVs during the biogenesis phase, which is characterized by the enrichment in some Lgps and that the kinetics of maturation of the SCV is retarded in cells overexpressing a dominant‐negative form of the rab7 GTPase.

Jan 11, 2019 · Detailed MS/MS assays, in vitro SUMOylation assays and structural docking analysis revealed SUMOylation of RAB7 (RAB7A) specifically at lysine 175. A SUMOylation-deficient RAB7 mutant (RAB7 K175R) displayed longer half-life, was beneficial to SCV dynamics and functionally deficient.

Jan 11, 2019 · Being a master regulator of endolysosomal trafficking, RAB7 activation and its context is crucial for the fate of the vesicle it belongs to, whether it is part of the late endosome or of the SCV. The precise location of RAB7 and its interacting partners determines these fates.

The following discussion will cover three topics: first, the GEF that activate Rab7/Ypt7p; second, the GAPs that inactivate Rab7/Ypt7p; and third, bacterial effectors, or virulence factors, that modulate Rab7 nucleotide binding to promote intracellular survival and pathogenesis.

Feb 3, 2004 · We found that the initial centripetal displacement of the SCV is due to recruitment by Rab7 of Rab7-interacting lysosomal protein (RILP), an effector protein that can simultaneously associate with the dynein motor complex.

Jan 14, 2019 · Detailed MS/MS assays, in vitro SUMOylation assays and structural docking analysis revealed SUMOylation of RAB7 (RAB7A) specifically at lysine 175. A SUMOylation-deficient RAB7 mutant...

We provide evidence for a functional synergism between PLEKHM1, HOPS, and Rab7 in regulating the SCV. The absence of any of these components causes substantial deformation of the SCV and limited proliferation of multiple Salmonella within a single, enlarged vacuole.

SCV maturation is characterized by selective interactions with the endocytic system. Bacterial vacuoles transiently acquire early and recycling endosome markers such as early endosomal antigen 1 (EEA1), transferrin receptor (TfnR), Rab5 and Rab11.