Mesd is a specialized chaperone for the low-density lipoprotein receptor-related protein-5 (LRP5) and LRP6. In our previous studies, we found that Mesd binds to mature LRP6 on the cell surface and blocks the binding of Wnt antagonist Dickkopf-1 (Dkk1) to LRP6.

In mammals, the RSPO protein family consists of four members, namely RSPO1 through RSPO4. They share approximately 40–60% amino acid sequence identity and substantial structural homologies within human and mouse members (Kazanskaya …

Sep 9, 2007 · Here we provide a mechanism whereby RSpo1 regulates cellular responsiveness to Wnt ligands by modulating the cell-surface levels of the coreceptor LRP6. We show that RSpo1 activity critically depends on the presence of canonical Wnt ligands and LRP6.

R-spondin-1 (Rspo1) is an intestinal growth factor known to exert its effects through activation of the canonical Wnt (cWnt) signaling pathway and subsequent expression of cWnt target genes. We have detected Rspo1 mRNA in murine islets and the murine MIN6 and βTC β-cell lines, and Rspo1 protein in MIN6 β-cells.

Jan 20, 2025 · RSPO1 enhances Wnt signaling by specifically stabilizing FZD5/8. Wnt promotes the interaction between FZD5 and RNF43. We further demonstrated that DVL proteins promote ligand-independent endocytosis of FZD but are dispensable for Wnt-induced FZD5/8 endocytosis and degradation.

May 6, 2021 · It has been shown that human Rspo1 is a high affinity ligand for LRP6, inducing GSK3-dependent phosphorylation and activation of LRP6 (Wei et al., 2007).

Sep 1, 2022 · RSPOs influence liver homeostasis and the development of multiple liver diseases. RSPO1 increases cell proliferation, protects hepatocytes from injury, improves liver regenerative potential, and affects liver metabolic zonation.

Jan 5, 2024 · The Co-IP results showed that treatment with RSPO1 significantly promoted the association of Axin1 with CK1ε and LRP6, suggesting that CK1ε may be involved in RSPO1-induced degradation of Axin1 (Fig. 3a). We next evaluated the role of CK1ε and CK1δ in the stability of the Axin1 protein.

Nov 14, 2013 · Rspo1 is involved in mammalian sex determination 4 and is a potent stimulator of epithelial repair in the gastrointestinal tract 2, 5. Rspo2 has recently been identified as a major determinant of...

Sep 11, 2007 · Here we provide a mechanism whereby RSpo1 regulates cellular responsiveness to Wnt ligands by modulating the cell-surface levels of the coreceptor LRP6. We show that RSpo1 activity critically depends on the presence of canonical Wnt ligands and LRP6.