Plasmid pAd-RFP from Dr. Tong-Chuan He's lab contains the insert RFP. This plasmid is available through Addgene.

Addition of an RFP marker for red-white selection in E. coli. The RFP is flanked by two BsaI sites. GG reaction leads to the loss of the RFP when the gene is inserted.

Red fluorescent protein (RFP) is a versatile biological marker for monitoring physiological processes, visualizing protein localization, and detecting transgenic expression in vivo. RFP can be excited by the 488 nm or 532 nm laser line and is optimally detected at 588 nm.

Plasmid pMXs GFP-LC3-RFP from Dr. Noboru Mizushima's lab contains the insert microtubule-associated protein 1 light chain 3 beta and is published in J Cell Biol. 2018 Aug 9. pii: jcb.201804132. doi: 10.1083/jcb.201804132. This plasmid is available through Addgene.

The Cas9-D10A nickase plasmid co-expressing RFP uses the CMV promoter for strong transient expression of Cas9-D10A. Alternate promoters can be substituted by replacement of CMV using MluI and NheI. Also, the Cas9D10A expression plasmids can be linearized using XbaI for T7-based mRNA production.

Customize plasmid maps with flexible annotation and visualization controls; Automatically generate a rich graphical history of every edit and procedure

The Sigma CRISPR dCas9p300 plasmid co-expresses p300-HAT and RFP, to easily monitor delivery and expression in your target cell type. gRNAs can successfully direct nuclease-deficient Cas9 (dCas9) fused to p300 HAT catalytic domain to increase levels of histone acetylation and endogenous gene expression.

To observe real-time interactions between green fluorescent protein-labeled immune cells and invading bacteria in the zebrafish (Danio rerio), a series of plasmids was constructed for the red fluorescent protein (RFP) labeling of a variety of fish and human pathogens.

Sep 11, 2015 · Plasmid pBbE6a-RFP from Dr. Jay Keasling's lab contains the insert RFP and is published in J Biol Eng. 2011 Sep 20;5:12. This plasmid is available through Addgene.

Use Sigma′s lentiviral Cas9 plasmid for generation of lentiviral particles and efficient production of stable cell lines expressing Cas9 for CRISPR based genome editing. Sigma′s Cas9-RFP lenti plasmid is one part of a two part CRISPR system with …