RAD51, a key component of the homologous recombination pathway, helps to stabilize DNA replication forks, repair the double strand break, and allow for the continuation of DNA synthesis. The AID-RAD51 Axis In Cancer: A Selective Synthetic Lethality Target

In humans, RAD51 is a 339- amino acid protein that plays a major role in homologous recombination of DNA during double strand break repair. In this repair process, an ATP-dependent DNA strand exchange takes place in which a template strand invades base-paired strands of homologous DNA molecules.

Rad51 recombinase is a critical effector of homologous recombination, which is an essential DNA repair mechanism for double-strand breaks. Rad51 has been found to be upregulated in many malignant solid tumors, and is correlated with poor prognosis. In multiple tumor types, Rad51 is critical for tumor metabolism, metastasis and drug resistance.

Jan 1, 2017 · RAD51-dependent repair is essential for recovery from radiation and temozolomide-induced DNA damage. Targeting this pathway may provide a tumor cell-specific effect in the context of chemo-radiotherapy.

RAD51 is an ATPase that forms a nucleoprotein filament on single-stranded DNA. RAD51 has the function of finding and invading homologous DNA sequences to enable accurate and timely DNA repair. Its paralogs, which arose from ancient gene duplications of RAD51, have evolved to regulate and promote RAD51 function.

We review here the multiple levels of regulation of RAD51 expression and function and explore potential therapeutic leads. The RAD51 recombinase is a critical effector of Homologous Recombination (HR), which is an essential DNA repair mechanism for double-strand breaks.

Jun 2, 2022 · Background: Homologous recombination (HR) is an essential, high-fidelity mechanism to repair DNA double strand breaks (DSBs) in cancer cells. CYT-0851 inhibits HR leading to an accumulation of unrepaired DSBs and tumor cell death.

Jul 2, 2020 · We show that RAD51 and other homologous recombination repair genes are overexpressed in metastatic melanoma cell lines and in melanoma patient samples, which correlates with reduced survival of...

RAD51 supports cell viability in vertebrates. Two-ended DSBs, such as those generated by ionising radiation, can be repaired by two major pathways: non-homologous end joining (NHEJ), which involves the direct ligation of the DNA ends to seal the break, and HR.

Jan 1, 2002 · To test whether Rad51 gene amplification causes up-regulation of Rad51 protein, we have visualized the Rad51 locus in eight representative tumor cell lines by metaphase FISH.