Science Biology Biology questions and answers Shown below is a polylinker that falls within the B-galactosidase coding region of the lacZ gene.

Question: Examine pUC19 polylinker site and the target DNA molecule then identify the best restriction sites to use to insert the provided DNA from the options given.

Question: 3. A polylinker site contains: a. sequences allowing linkage to mRNA. b. a sequence that allows for insertion into the host chromosome. c. many closely spaced restriction enzyme sites. d. a sequence that cannot be cleaved. e. links for antibiotic resistance. 4. What pair of amino acids is positively charged at a neutral pH? a. Cys, Met b. Leu, Pro c. His,

Question: 5. Overlapping restriction sites in a plasmid vector polylinker The nucleotide sequence of a polylinker in a particular plasmid vector is 5'-GAATTCCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGC-3' This polylinker contains restriction sites for BamHI, EcoRI, Pstl, Sall, Smal, Sphl, and Xbal.

It has a Polylinker site, also called a multiple cloning site (MCS) where a gene of interest can be added so bacteria can express it as protein. The sequence of the MCS is show with the location of the restriction sites.

1) A polylinker (also known as a multiple cloning site) is a: A) synthetic DNA fragment that contains multiple restriction enzyme sites B) synthetic DNA fragment inserted into a vector that allows replication of the vector in the host cell C) DNA sequence that allows for selection of cells that contain the vector D) molecule used to ligate DNA fragments together E) synthetic DNA fragment used ...

What is the role of a polylinker or multi-cloning site in a plasmid used for cloning in E coli? So that the plasmid is maintained in the E coli cells as they grow and divide.

Cloning vectors often have polylinkers consisting of a multiple array of restriction sites at their cloning sites, so restriction fragments generated by a variety of endonucleases can be incorporated into the vector. Note that the polylinker is engineered not only to have multiple restriction sites but also to have an uninterrupted sequence …

Question: In the pUC plasmids, a polylinker region that includes multiple restriction sites is embedded in the lacZ component. If a sequence of interest is inserted into this region, what is the effect on lacZ?

Science Biology Biology questions and answers 4. When a foreign gene is inserted into the PMAL plasmid at the Xmn I site in the polylinker and the modified PMAL is transformed into E. coli bacteria, the E. coli will use the inserted gene to make large amounts of the foreign protein. Draw a sketch of the PMAL plasmid to show where a foreign gene DNA should be inserted in the plasmid in order to ...