Since its introduction in plants 10 years ago, the bimolecular fluorescence complementation (BiFC) method, or split-YFP (yellow fluorescent protein), has gained popularity within the plant biology field as a method to study protein-protein interactions.

The unicellular green alga Chlamydomonas reinhardtii is a valuable experimental system in plant biology for studying metal homeostasis. Analyzing transcriptional regulation with promoter-fusion constructs in C. reinhardtii is a powerful method for ...

Fluorescence emission spectra of intact, BiFC reconstituted YFP and autofluorescence of plant tissues. Samples of YFP (yellow line) and ROP-ICR1 (green line) were excited at 488 nm and the fluorescent spectra were determined using the spectral scanning module …

Mar 22, 2019 · Whole seedlings of transgenic plant lines expressing PROPEP1-YFP (yellow fluorescent protein) fusion proteins were damaged with serrated forceps or by thawing of snap-frozen and crushed tissue . In both experimental setups, we evaluated the PROPEP1-YFP cleavage kinetics through immunoblot analysis.

Jun 26, 2006 · Non-invasive fluorophore-based protein interaction assays like fluorescence resonance energy transfer (FRET) and bimolecular fluorescence complementation (BiFC, also referred to as "split YFP") have been proven invaluable tools to …

Dec 6, 2007 · Following the complete genome sequencing of different plant species such as Arabidopsis (Arabidopsis thaliana), rice (Oryza sativa), and Physcomitrella (Physcomitrella patens), as well as advances toward deciphering entire proteomes, the need for a reliable way to identify protein-protein interactions is becoming a major task for the future.

Oct 6, 2006 · We demonstrate the utility of these vectors for the analysis of specific protein–protein interactions in various cellular compartments, including the nucleus, plasmodesmata, and chloroplasts of different plant species and cell types.

Here we report the determination of protein-protein interactions in plants using bimolecular fluorescence complementation (BiFC). The yellow fluorescent protein (YFP) was split into two non-overlapping N-terminal (YN) …

Nov 29, 2019 · We developed a colorimetric YFP assay and then quantified changes in YFP concentrations in OPR3 promoter:YFP-PTS1 transgenic plants in response to treatments with caterpillar herbivory and insect feeding vibrations.

In order to extend our understanding of the spatial aspect of the plant immune system, we generated transgenic Arabidopsis plants expressing yellow fluorescent protein (YFP) fused to the...