Jul 13, 2021 · A recent study confirmed the timing of major ZGA, employing the single-cell RNA sequencing (scRNA-seq) method on pig early in vivo fertilized embryos, to dissect the molecular features of ...

Nov 24, 2022 · In the present study, we revealed the transcriptome profiles of porcine IVV and SCNT embryos and performed cross-species analysis of transcriptional activity during ZGA in pigs, mice, bovines and humans.

Jan 2, 2025 · The zygotic genome activation (ZGA) is crucial for the development of pre-implantation embryos. Long noncoding RNAs (lncRNAs) play significant roles in many biological processes, but the study on their role in the early embryonic development of pigs is limited.

As expect, we found that the modules in the 4- to 8-cell transition corresponding to zygotic genome activation (ZGA) in pig were enriched for transcription regulation, epigenetic regulation and cell cycle (Fig. 4C and see Supplementary Table S3).

Nov 24, 2022 · Pigs and bovines have the highest coefficient of similarity in biological processes during ZGA. This study provides an important reference for further studying the reprogramming regulatory mechanism of porcine embryos during ZGA.

Genome-wide transcriptome analysis revealed unique molecular gene regulation networks which regulate ZGA, lineage segregation and embryonic metabolism during pig PED. Some highly expressed genes in pig putative ICM, most of which are related to ES cell pluripotency, differed from those highly expressed in mouse ICM [ 18 ].

Sep 19, 2024 · In pigs, ZGA occurs at the 4-cell (4 C) stage, indicating that correct gene expression at this stage plays an important regulatory role in embryonic development. However, the effect of...

The zygotic genome activation (ZGA) is crucial for the development of pre-implantation embryos. Long noncoding RNAs (lncRNAs) play significant roles in many biological processes, but the study on their role in the early embryonic development of pigs is limited.

These results suggest that ELF4 affects ZGA and embryonic development competency in porcine embryos by maintaining genome integrity and regulating dynamic changes of H3K9me3 and DNA methylation, and correctly activating ZGA-related genes to promote epigenetic reprogramming.

Jul 13, 2021 · We found that minor and major ZGAs occur at 1-cell and 4-cell stages for both IVF and PA embryos, respectively. Maternal RNAs gradually decay from 1- to 8-cell embryos.