Intracellular pH (pH i) is the measure of the acidity or basicity (i.e., pH) of intracellular fluid. The pH i plays a critical role in membrane transport and other intracellular processes. In an environment with the improper pH i, biological cells may have compromised function.

Explore the role of pH in cell health. pHrodo pH indicators are ideal live-cell probes for the study of intracellular pH and cellular internalization, and their regulation in both normal development and disease processes.

An E. coli cell has a cytoplasmic pH of ≈7.2-7.8 (BNID 106518) measured as discussed below. This pH value is a result of both active and passive mechanisms required for the ability of E. coli to colonize the human gastrointestinal tract that contains niches of …

The aim of this review is to summarize and critically discuss the possible roles of pH i and pH e in cell cycle progression. While the focus is on the mammalian cell cycle, important insights from studies in lower eukaryotes are also discussed.

Cell pH refers to the pH level inside a cell, which is approximately 7.0 and slightly different from the pH of the extracellular fluid. It plays a crucial role in determining the distribution and accumulation of weak electrolytes and can significantly impact the dose response of …

Apr 26, 2019 · Here, we use real-time measurements of medium pH and intracellular pH under live-cell culture conditions to describe the effects of various buffering regimes, including physiological CO 2...

Dec 9, 2009 · Cellular compartments are protected from rapid, localized pH swings by their inherent pH buffering capacity (β). The total buffer capacity (β total) consists of two components: β intrinsic and...

Oct 25, 2011 · These compartment-dependent changes in pH can be visualized in living cells by using pH-sensitive fluorescent probes, nuclear magnetic resonance, or pH microelectrodes, but their assessment and modulation in vivo remains a challenge.

Intracellular pH is a vital parameter that is maintained close to neutrality in all mammalian cells and tissues and acidic in most intracellular compartments. After presenting the main techniques used for intracellular an vesicular pH measurements ...

Unlike intracellular free Ca 2+ concentrations, which can rapidly change by perhaps 100-fold, pH inside a cell varies by only fractions of a pH unit, and such changes may occur quite slowly. We offer a range of indicators for tracking intracellular pH in the cytosol or in particular organelles.