Bacterial vector for expression of N-terminally S-tagged proteins with an enterokinase site. For other reading frames, use pET-30b (+) or pET-30c (+). Sequence Author: MilliporeSigma …

produce virions containing single-stranded DNA that corresponds to the coding strand. Therefore, single-stranded sequencing should be performed using the T7 terminator primer (Cat. No. 69337-3).

General Plasmid Transform Protocol. 1. Take one 100μl of the competent cells and thaw it on ice for 10min, add 2μl of plasmid, then ice bath for 30min, then heat-shock it at 42℃ for 60s, do …

What strain of bacteria does my stab contain? How can I be notified when a new material becomes available? What is virus associated DNA, and why do I have to order it? Welcome to Vector Database! Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.

pET-30a(+) DNA - Novagen - a vector enabling expression of proteins with N-terminal His•Tag®, thrombin, S•Tag and enterokinase sequences, plus an optional C-terminal His•Tag.

Plasmid pET-30a (+)-N-SH2 from Dr. Carol Post's lab contains the insert murine Syk (N)SH2 domain (Ser 8 to Gly 117), with an N-terminal (His)6 tag and TEV cleavage site and is published in Phys Chem Chem Phys. 2016 Feb 17;18 (8):5807-18. doi: 10.1039/c5cp05417f. This plasmid is available through Addgene.

pET30a, pET30a-plus. Bacterial expression vector with T7 promoter; kanamycin resistance; restriction enzyme cloning. None. 5400. None. ''

pET-30a (+) DNA - Novagen MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents. The pET-30a-c (+) vectors carry an N-terminal …

Aug 25, 2011 · </protect> Notes History You can help EcoliWiki by writing about the history of this plasmid. Sources Novagen References Sequence 1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa 61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt 121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt 181 cgacggagct cgaattcgga tccgatatca gccatggcct tgtcgtcgtc ...

The pET-30a-c (+) vectors carry an N-terminal His·Tag™/thrombin/S·Tag™/enterokinase configuration plus an optional C-terminal His·Tag sequence. Store at -70°C. Avoid freeze/thaw …