Plasmid pCHA-VRC01-scFv from Dr. Dane Wittrup's lab contains the insert VRC01 scFv and is published in J Mol Biol. 2013 Jan 23;425 (2):444-56. doi: 10.1016/j.jmb.2012.11.010. Epub 2012 Nov 15. This plasmid is available through Addgene.

Plasmid pCHA_SBV_NSs from Dr. Gerald Barry's lab contains the insert NSs and is published in Unpublished This plasmid is available through Addgene. Image: Illustrated plasmid map in PNG format GenBank File: Plasmid sequence and annotations.

Plasmid pCHA1.1-Rap2a-V5-Hygro from Dr. Joanna Wysocka's lab contains the insert Hygromycin and is published in Science. 2023 Aug 11;381 (6658):eade6289. doi: 10.1126/science.ade6289. Epub 2023 Aug 11. This plasmid is available through Addgene.

In this work, we demonstrate that the Methanomethylophilus alvus PylRS (MaPylRS) and its cognate tRNA CUAMaPyl support the incorporation of ncAAs into proteins produced in S. cerevisiae using stop codon suppression methodologies.

Mar 20, 2023 · The correct insertion and sequences of the P pchA promoter in the promoter test vectors, lacI in the propionate biosensor, and the mGM-CSF gene in therapeutic response plasmids were verified through plasmid sequencing at Macrogen, Inc.

Yeast display plasmids for displaying WT TIMP-1 and TIMP-1 mutants at the N terminus of Aga2p protein on the yeast surface were derived from the pCHA backbone, pCHA-VRC01 vector purchased from Addgene.

Sep 22, 2022 · These results indicate that SlyA is a positive regulator of pchA and is independent of the Lrp regulatory system. SlyA may be involved in the virulence expression in EHEC, maintaining a certain level of expression in the absence of a butyrate response.

Sep 20, 2016 · Prey genes were digested with restriction enzymes (EcoRI/XhoI) and cloned into the mammalian expression vector pcHA. Insertion of the prey genes was confirmed by restriction enzyme digestion and nucleotide sequencing ( Fig. 1E ).

Oct 13, 2017 · Plasmid pET28b-pchA WT from Dr. Audrey Lamb's lab contains the insert pchA WT and is published in Arch Biochem Biophys. 2013 Oct 1;538(1):49-56. doi: 10.1016/j.abb.2013.07.026. Epub 2013 Aug 11. This plasmid is available through Addgene.

Apr 29, 2022 · These restriction enzymes are for the pCTcon2 plasmid available through Addgene. This plasmid contains CD20 as the insert and the backbone can be linearized by triple digestion. The pCHA plasmid on Addgene contains VRC01 scFv and should be linearized with only Nhe I-HF and Bam HI-HF (not Sal I-HF).