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Plasmid pCAG-GFP from Dr. Connie Cepko's lab contains the insert EGFP from Aequorea victoria and is published in Proc Natl Acad Sci U S A. 2004 Jan 6. 101 (1):16-22. This plasmid is available through Addgene.

The pCAGGS vector has several distinctive features. 1. It has a unique promoter combination of chicken β-actin promoter and CMV enhancer (CAG promoter), enabling high-level expression in a wide range of cell types and being more versatile than some single strong promoters. 2. It shows low cytotoxicity and high genetic stability. 3.

pCAG is a gene expression vector for mammalian cells. pCAG includes CAG promoter in itself. CAG promoter is known to be able to artificially induce high expression of target recombinant proteins in mammalian cells. pCAG-Neo PA tag is inserted PA tag into N- or C-terminal part of multiple cloning site(MCS) in pCAG-Neo vector.

The Procurement Customer Advisory Group helps guide Washington state procurement strategy for goods and services. Originally formed to help DES implement procurement reform, the group is composed of senior procurement managers from a representative group of state agencies and institutions of higher education.

Differentiating congenital type I protein C deficiency from type II deficiency.

Based on next-generation sequencing (NGS) results where indicated (Addgene NGS Result), or assembled from reference sequences and/or Sanger results (Addgene Assembled Sequence). Addgene has sequenced portions of this plasmid for verification. The results are shown below. Leading primers are indicated on the first line of each sequence.

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Plasmid pCAG-Cre from Dr. Connie Cepko's lab contains the insert Cre and is published in Proc Natl Acad Sci U S A. 2007 Jan 16;104 (3):1027-32. doi: 10.1073/pnas.0610155104 This plasmid is available through Addgene.

Testing of protein C functional activity (CFX / Protein C Activity, Plasma) is recommended for initial laboratory evaluation of patients suspected of having congenital protein C deficiency …