Jun 8, 2015 · Protein kinase C (PKC) inhibitors also blocked ES-induced ROS production. Our results indicate that physiological levels of electrical stimulation induce ROS production in skeletal muscle cells through release of extracellular ATP and activation of P2Y1 receptors.

During micropinocytosis of antigens in DC, diacylglycerol (DAG)-inducible PKC-δ stimulation drives NOX2 activity . During phagocytosis, members of the PKC-family are phosphorylated and activated by phagocytic receptor-mediated stimulation of SYK .

PKC can also activate Nox2. In human monocytes and murine macrophages PI3K and PKC pathways are involved in Nox2 stimulation in response to insulin. This mechanism triggers ERK1/2, p38MAPK and NFκB, which culminates in the activation of monocytes and proliferation of macrophages . PKC-dependent Nox2 activation was also shown in coronary artery ...

Endothelium-denuded bovine coronary arteries showed a PKC-dependent basal production of lucigenin (5 muM)-detected Nox oxidase-derived superoxide, which was stimulated fourfold by PKC activation with 10 muM phorbol 12,13-dibutyrate (PDBu).

Although the activation and plasma membrane translocation of protein kinase C (PKC) isoforms in dorsal root ganglion (DRG) neurons have been implicated in multiple pain models, the interactions between NOX2-induced oxidative stress and PKC remain unknown.

Jan 23, 2009 · Activation of Nox2-derived peroxide generation by a p47 phox-dependent mechanism appears to be a key component of the contraction elicited by PDBu promoting PKC activation in both BCA and mouse aorta.

Jun 3, 2008 · In the present study, we clearly show that gp91 phox /NOX2 is phosphorylated during activation of human neutrophils, we provide evidence that protein kinase C (PKC) is involved in this process, and we show that phosphorylation potentiates intrinsic diaphorase activity of gp91 phox /NOX2 and interaction with Rac, p67 phox, and p47 phox.

Jun 8, 2015 · We hypothesized that ATP extruded from the muscle fiber increases ROS production via PKC-NOX2. Our results indicate that both electrical stimulation and extracellular ATP induced ROS production in skeletal muscle cells, …

Mar 26, 2018 · PKCδ/Nox2-mediated antigen macropinocytosis stimulates maturation of DCs and secretion of T-cell stimulatory cytokines. These findings may contribute to a better understanding of the regulatory mechanisms in DC macropinocytosis and downstream regulation of T-cell-mediated responses.

Nov 1, 2014 · The subsequent activation of Syk stimulates activation of PLCγ1, PKC and eventually Nox2 [226]. Sphingosine-1-phosphate contributes to fMLP-induced Nox2 activation by controlling intracellular calcium [227] but in fibroblasts [228] and arteries [229] S1P also directly activates NADPH oxidases.