4 days ago · Western blot analysis of extracts from SW-13, NIH/3T3, Jurkat and PC12 cells, using PDK1 Antibody. Cat. Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody. PDK1 Antibody detects endogenous levels of total PDK1 protein.

Apr 8, 2025 · Polyclonal Antibody for studying PDK1 (Ser241) phosphate. Validated for IP, WB. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-PDK1 (Ser241) Antibody (CST #3061) is ready to ship.

10026-1-AP targets PDK1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples. PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for …

Phospho-PDK1 (Tyr373/376) Antibody detects endogenous levels of PDK1 when only phosphorylated at tyrosine 373/376. The antibody may cross-react with some other proteins. CST recommends an immunoprecipitation of PDK1 prior to Western immunoblotting.

PDK1 (human, recombinant): Active • Host: Insect cells • AA: 1-556 (full length) • Tag: N-terminal His • MW: 66.7 kDa. Synonym: 3-Phosphoinositide-dependent Protein Kinase 1. Purity: ≥85% estimated by SDS-PAGE.

PDK1 inhbits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism. PDK1 antibodies are useful tools for glucose metabolism research and mitochondrial metabolism studies.

Jun 6, 2023 · Monomeric full-length PDK1 1-556 had D max = 110 Å and Rg = 34.8 Å, with the MW estimated between 67 and 79 kDa by different calculation approaches (Table 1).

Recombinant full-length house PDK1 was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag. The gene accession number is NM_002613. Figure 1. SDS-PAGE gel image. The purity of PDK1 was determined to be >85% by densitometry. Approx. MW 67kDa.

Phospho-PDK1 (Ser241) Antibody is a rabbit antibody raised against a short peptide from N human PDK1 sequence surrounding and containing phospho-Ser241. This antibody detects endogenous phosphorylated PDK1 (Ser241) proteins …

PDK1 has 2 isoforms with the MW of 49 and 52 kD, and a 28aa-transit-peptide in the N- terminal can be removed in the mature form. This antibody is specific to PDK1.