Three major CD33 (+) subsets including CD33 (bright)CD14 (bright) Mo, CD33 (bright)CD14 (-) CD11c (+) mDC and CD33 (dim)CD14 (-) pDC were present. Dendritic cells subsets and Mo expressed low levels of CC and CXC receptors, but distinctive chemokine receptor expression profiles were not observed.

Death Receptor 6 (DR6; TNFRSF21/CD358) is a specific marker of human pDC and knock‐down in pDC cell lines impairs IRF7 translocation to the nucleus. 72 Sphingosine‐1‐phosphate signalling interacts with ILT7 to limit TLR‐induced interferon production 73 and with IFNAR to terminate the IFN‐ α response. 74

Here, we combine two high-dimensional technologies—single-cell messenger RNA sequencing (scmRNAseq) and cytometry by time-of-flight (CyTOF)—to identify human blood CD123 + CD33 + CD45RA + DC precursors (pre-DC). Pre-DC share surface markers with plasmacytoid DC (pDC) but have distinct functional properties that were previously attributed to ...

Apr 14, 2022 · We identified CD9 + nonclassical monocytes and CD33 hi classical monocytes as two populations that are positively correlated with ICB responsiveness. The use of machine learning recapitulates the importance of CD33 expression on monocytes during anti-PD-1 therapy.

May 29, 2019 · Conventional DCs (cDCs) and plasmacytoid DCs (pDCs) are distinguished by morphology and function, and can be easily discriminated by surface marker expression, both in mouse and man.

Apr 14, 2022 · Our use of machine learning corroborated the association of specific monocyte markers with responsiveness to ICB. Our work provides a high-dimensional profile of monocytes in NSCLC and links CD33 expression on monocytes with anti …

Briefly, PBMC-tumor cell line co-cultures were established in tissue culture flasks for one week. Tumor-educated myeloid (CD33 +) cells were then isolated, checked for viability, and tested for suppressive function by co-culture with fresh, autologous T cells in the presence of T cell stimuli.

CD33 or Siglec-3 (sialic acid binding Ig-like lectin 3, SIGLEC3, SIGLEC-3, gp67, p67) is a transmembrane receptor expressed on cells of myeloid lineage. [5] It is usually considered myeloid-specific, but it can also be found on some lymphoid cells.

These APC populations include myeloid dend-ritic cells (mDC), plasmacytoid DC (pDC) and monocytes (Mo). To assign spe-cialized functional roles and stages of development for APCs, CD33 expressing APC subsets were examined for their capacity to respond to chemokines.

Feb 1, 2005 · Overall, our results demonstrate that the expression of CD33 on CD4 (+)CD56 (+) lineage-negative cells should not exclude the diagnosis of pDC leukemia and underline that pDC-specific markers should be used at diagnosis for CD4 (+)CD56 (+) malignancies.