Feb 14, 2018 · Probe and primer are two types of single-stranded oligonucleotides used in various types of PCR. Probes are used in the detection of specific DNA fragments in qPCR. Primers are used to initiate DNA replication inside the cell and they are also used in the initiation of PCR.

Among the most popular PCR probes out there are hydrolysis probes, Molecular Beacon probes, dual hybridization probes, Eclipse probes, Scorpion probes, and a few others. There are different types of PCR probes commercially available.

Oct 21, 2013 · Many factors can influence PCR experiments, including primer and probe characteristics such as location, length, interaction and self-folding, melting temperature, annealing temperature, and GC content. Review these general recommendations for designing primers and probes and for choosing target locations for PCR amplification.

Learn about PCR primer and probe chemistries for real-time PCR. Discover DNA primers with fluorescent DNA-binding dyes, hydrolysis probes and molecular beacons.

Apr 7, 2015 · Learn what fluorophores are compatible with various real-time PCR instruments and best practices for picking reporter dyes and quenchers on qPCR probes.

A real-time polymerase chain reaction (real-time PCR, or qPCR when used quantitatively) is a laboratory technique of molecular biology based on the polymerase chain reaction (PCR). It monitors the amplification of a targeted DNA molecule during the PCR (i.e., in real time), not at its end, as in conventional PCR. Real-time PCR can be used ...

Review options for custom-synthesized oligos, primers, probes, and genes for PCR, cloning, hybridization, real-time PCR, protein expression. Also, access our easy oligo ordering portal or primer and probe design tools.

Nov 9, 2017 · PCR (Polymerase Chain Reaction) is a revolutionary method developed by Kary Mullis in the 1980s. PCR is based on using the ability of DNA polymerase to synthesize new strand of DNA complementary to the offered template strand.

Probe-based quantitative PCR (qPCR) uses real-time fluorescence from 5ʹ-3ʹ exonuclease cleavage of a fluorescently-labeled, target-specific probe to measure DNA amplification at each cycle of a PCR.

PCR probes are DNA or RNA sequences labeled with a reporter molecule. The reporter is a molecule that emits fluorescence when hybridized to the target sequence. These probes are used in quantitative and real-time PCR methods such as qPCR or quantitative PCR, which allows real-time amplification detection during the PCR process.