We showed that cell proliferation was substantially restricted by S-phase arrest and apoptosis in the MAGT1-knocked down cells, which was further confirmed by the increased expression of p21, cyclin-A1, and cyclin-B1, as well as the decreased expression of …

Apr 13, 2020 · Here, we demonstrate for the first time that stably decreasing cGAS expression levels in different cancer cell lines (HeLa and U2OS) facing nocodazole-induced mitotic arrest induces chromosomal...

Oct 9, 2024 · To demonstrate the role of this box in p21 degradation, we edited the genome of HEK293 and HeLa cell lines using a CRISPR strategy. We found that the p21 half-life increased in cells with either a deleted or mutated p21 RRLIF box.

We found a significant loss of p21 mRNA expression in RNASEL−/− MEFs and that the overexpression of RNase L in HeLa cells induces p21 mRNA expression. The p21 mRNA half-life significantly changes as a result of RNase L modulation, indicating a post-transcriptional effect.

p21 Waf1/Cip1 protein levels respond to DNA damage; p21 is induced after ionizing radiation, but degraded after UV. p21 degradation after UV is necessary for optimal DNA repair, presumably because p21 inhibits nucleotide excision repair by blocking proliferating cell …

HeLa cells were infected with either Ad.null (an adenovirus carrying no insert) or Ad.p21 (an adenovirus expressing a p21 cDNA), with a virus dose of 100 plaque-forming units (pfu)/cell (sufficient to infect greater than 90% of the cell population).

Dec 3, 2012 · In HeLa cells, p21 messenger-RNA (mRNA) increased concomitantly but the level of p53 mRNA was unaffected. RNA interference directed against p53 prevented the induction of both proteins.

We found a significant loss of p21 mRNA expression in RNASEL(-/-) MEFs and that the overexpression of RNase L in HeLa cells induces p21 mRNA expression. The p21 mRNA half-life significantly changes as a result of RNase L modulation, indicating a post-transcriptional effect.

Western Blot analysis showed that the level of p21 expression in treated Hela and U2OS cells is higher compared to the controls ( Figure 5). This suggests the involvement of p21 protein in...

Apr 8, 2021 · We showed that cell proliferation was substantially restricted by S-phase arrest and apoptosis in the MAGT1-knocked down cells, which was further confirmed by the increased expression of p21, cyclin-A1, and cyclin-B1, as well as the decreased expression of MYC, cyclin-D1, cyclin-E1, and CDK2.