In this study, we demonstrated a bio-ligation approach to display single-chain variable fragments (scFv) on the OMV surface using the SpyTag/SpyCatcher system. SpyTag-fused scFv, …

We demonstrate that E. coli display using an N‐terminal domain of intimin is an effective platform for the surface display of VHH libraries enabling selection of high‐affinity Nbs by magnetic cell …

Oct 13, 2016 · By the external exposed loops of the C-terminal of OmpA, scFv which designed at behind of Lpp-OmpA can be easily anchored to the outer membrane. Between the OmpA and …

Dec 1, 2006 · In this report, we describe the use of the pJB658-based expression system (26) to produce GM-CSF, IFN-α2b, and scFv-phOx both intracellularly (lacking signal sequences) and …

scFv expression can be directed to either the oxidizing environment of the periplasmic space, under regulation of the pelB leader sequence (Padiolleau-Lefèvre et al., 2006), or to the culture ...

Jul 1, 1999 · The surface display of single-chain antibody (scFv) in Escherichia coli as an Lpp–OmpA′ fusion was monitored using a fluorescently tagged antigen in conjunction with flow …

We have studied the influence of bacterial host on the secretion of single-chain Fv antibody fragment (scFv), the production of this antibody fragment as intracellular fusion protein, and …

The features of Lpp-OmpA expression system. The versatile applications： The diversity of introduced protein connecting to OmpA can provide the multiple functions outside the surface …

Sep 3, 2024 · In this study, we demonstrated a bio-ligation approach to display single-chain variable fragments (scFv) on the OMV surface using the SpyTag/SpyCatcher system. SpyTag …

Based on the aforementioned studies, we proposed the expression of high-affinity CEA-specific single chain antibody fragments (scFv) into use on the surface of the bacteria. Figure 1: The …