Jul 1, 2020 · Primary microglia cells expressing GFP or GFP-tagged Nurr1 were treated with LPS (0.2 μg/ml) for 3 h to induce the inflammatory response. We analyzed the expression level of the proinflammatory genes including IL-1β, IL-6, and TNFa, microglial cell …

Jul 3, 2020 · The Nurr1–NCoR-1 interaction was studied by titrating GFP-Nurr1 LBD against biotinylated NCoR-1 copeptide (18 nM) and Tb-SA (12 nM) in presence of a fixed concentration (100 µM, in assay...

Our work identifies the Lem2-Nur1 complex as a substrate for the nuclear ESCRT machinery and explains how the dynamic tethering of chromosomes to the INM is linked to the establishment of nuclear compartmentalization. Keywords: nuclear envelope, ESCRT-III, Lem2, heterochromatin, fission yeast, Vps4.

Jan 2, 2020 · To address the possible role of Lem2-Nur1 at the rDNA repeats in fission yeast, we examined the localization of Cnp1-GFP and Gar1-GFP in wild-type, lem2Δ, nur1Δ, lem2Δnur1Δ, and clr4Δ cells in which the nuclear envelop was marked with Cut11-mKO2.

Jul 29, 2022 · Intra-cranial delivery of Nurr1 and Foxa2 genes using adeno-associated virus (AAV) serotype 9 improved the memory and cognitive function of AD model mice. The therapeutic benefits of gene delivery...

Jul 28, 2022 · Here, we analyze Nurr1's structure and mechanisms compared to other nuclear receptors, summarize the known small molecule Nurr1 ligands, and discuss the available evidence for the therapeutic potential of Nurr1 in neurodegeneration.

Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information.

NURR1 (Nuclear receptor-related 1 protein or NR4A2) is a nuclear protein receptor transcription factor with an essential role in the development, regulation, and maintenance of dopaminergic neurons and mediates the response to stressful stimuli during the perinatal period in mammalian brain development.

Jul 1, 2020 · To provide new insights into the molecular mechanisms of Nurr1 in the inflammatory response, we performed Chromatin immunoprecipitation sequencing (ChIP-Seq) on LPS-induced inflammation in BV2...

Apr 3, 2019 · Deconvolved inverted fluorescence micrographs of Heh1-mCherry (red channel), Heh2-mCherry (red channel) and Nur1-GFP (green channel) are shown in indicated columns in both the top and bottom panels.