NLR family pyrin domain containing 3 (NLRP3) (previously known as NACHT, LRR, and PYD domains-containing protein 3 [NALP3] and cryopyrin), is a protein that in humans is encoded by the NLRP3 gene [5] located on the long arm of chromosome 1. [6]

Dec 18, 2023 · Nucleotide-binding domain, leucine-rich repeat, and pyrin domain–containing protein 3 (NLRP3), which belongs to NLR family, can be activated by a broad range of microbial components, endogenous...

Jul 7, 2021 · Nlrp3-mediated activation of caspase 1 promotes proteolytic cleavage and maturation of two pro-inflammatory cytokines, interleukin-1β (IL-1β) and interleukin 18 (IL-18), and cleavage of gasdermin D, which forms cell membrane pores involved in secretion of mature IL-1β and IL-18 [12 – 14].

In this review, we summarize our current understanding of the mechanisms of NLRP3 inflammasome activation by multiple signaling events, and its regulation by post-translational modifications and interacting partners of NLRP3.

Nlrp3 can act as a transcription factor in T cells by binding directly to the Il4, Il13, Icos, Maf, Il1r1 promoter region. Nlrp3 can induce phosphorylation of Smad2/4 in the TGFβR pathway, thereby regulating the transcription of target genes.

Jan 14, 2025 · The NLRP3 inflammasome is a cytosolic complex that recognizes pathogenic insults and cellular perturbations. 1 Its activation typically requires two signals.

Mar 30, 2025 · NLRP3 (NLR Family Pyrin Domain Containing 3) is a Protein Coding gene. Diseases associated with NLRP3 include Muckle-Wells Syndrome and Cinca Syndrome. Among its related pathways are SARS-CoV-2 Infection and Inflammasomes. Gene Ontology (GO) annotations related to this gene include peptidoglycan binding. An important paralog of …

Oct 6, 2021 · We used mass spectrometry in order to identify novel modification sites in the LRR domain of NLRP3 that could be linked to ubiquitination. 293T cells expressing Flag-NLRP3 LRR were treated with...

Jul 25, 2018 · Here we show that the synthesis of mitochondrial DNA (mtDNA), induced after the engagement of Toll-like receptors, is crucial for NLRP3 signalling. Toll-like receptors signal via the MyD88 and TRIF...

绿色：NLRP3，红色：alpha Tubulin，蓝色：DAPI。 NLRP3在正常组织中表达量很低，例如小鼠肝脏、肾脏组织等。 检测前需要确认靶标蛋白表达量，同时建议选取抗体产品说明书推荐的阳性对照一同开展实验。 对于部分细胞如RAW264.7，LPS 处理会增加胞内NLRP3的表达，因此LPS刺激处理有利于解决WB实验时无信号或信号弱等问题。 (可参考阳性对照) 在WB实验中，NLRP3可能会检测到多条条带，因为在人和小鼠中，NLRP3有多个异构体。 由于NLRP3的不同修饰形 …