Oct 8, 2021 · Adenine base editors (ABE) are genome-editing tools that have been harnessed to introduce precise A•T to G•C conversion. However, the low activity of ABE at certain sites remains a major...

Nov 1, 2017 · Here we describe adenine base editors (ABEs) that mediate the conversion of A•T to G•C in genomic DNA. We evolved a transfer RNA adenosine deaminase to operate on DNA when fused to a...

To develop a CBE that retains the small size, low off-target activity and high on-target activity of current ABEs, we evolved the highly active deoxyadenosine deaminase TadA-8e to perform...

Finally, we show that ABE8e can efficiently install natural mutations that upregulate fetal hemoglobin expression in the BCL11A enhancer or in the the HBG promoter in human cells, targets that were poorly edited with ABE7.10. ABE8e augments the effectiveness and applicability of adenine base editing.

In vivo base editing rescued the vascular pathology of the mice, preserving vascular smooth muscle cell counts and preventing adventitial fibrosis. A single injection of ABE-expressing AAV9 at postnatal day 14 improved vitality and greatly extended the median lifespan of the mice from 215 to 510 days.

Oct 7, 2021 · Here we report adenine base editors (ABEs) that mediate conversion of A•T to G•C in genomic DNA. We evolved a tRNA adenosine deaminase to operate on DNA when fused to a catalytically impaired CRISPR-Cas9.

2019年10月21日，哈佛大学David Liu实验室在 Nature 杂志上发表了题为 Search-and-replace genome editing without double-strand breaks or donor DNA 的论文。 文章开发出了全新的精准基因编辑工具PE （Prime Editors），新工具PE无需额外的DNA模板便可有效实现所有12种单碱基的自由转换，而且还能有效实现多碱基的精准插入与删除（最多可插入44bp的碱基，可删除80bp的碱基），这一全能性的工具为基因编辑领域带来了重大变革。

Adenine base editors (ABEs) catalyze A-to-G transitions showing broad applications, but their bystander mutations and off-target editing effects raise safety concerns.

Aug 1, 2022 · A hypercompact adenine base editor termed TaRGET-ABE was developed by fusing a catalytically inactive transposon-associated transposase B guided by an engineered RNA to deaminases, which achieves...

总体而言，两项研究均表明，利用脂质纳米颗粒LNPs递送单碱基编辑工具ABE可实现非人灵长类动物肝脏中PCSK9基因的高效精准编辑，而且只需单次注射便可实现血液中PCSK9和LDL胆固醇的持久降低，这为心血管疾病的预防和治疗提供了全新的思路。 这是研究者首次在非人灵长类动物体内开展单碱基编辑治疗的研究，具有非常重要的指导意义，将极大的推动单碱基编辑技术的临床转化应用。 1. R Gaudelli, N. M. et al. Programmable base editing of A•T to G•C in genomic DNA …