Expression of hmox1 and nqo1 was measured using real-time PCR and cellular accumulation of Nrf2 was assessed by Western blot. Our results showed an increased expression at early time points of hmox1 and nqo1 mRNAs in response to sensitizers but not to irritants.

One of the genes regulated through Nrf2 is heme oxygenase-1 (HO-1, HMOX1, EC 1.14.99.3) . HO-1 is one of two distinct HO isoforms found in mammals. It serves as an inducible 32-kDa protein, highly upregulated by a number of stimuli like heme, nitric oxide, heavy metals, growth factor, cytokines, modified lipids and others.

Jul 22, 2019 · NQO1, HO-1, and MT1A involve in the regulation of redox homeostasis. Expressions of NQO1, HMOX1, and MT1A are under ARE on their gene promoter, suggesting their involvement in the same redox regulation pathway.

Next to NAD(P)H dehydrogenase [quinone] 1 (gene: nqo1; protein: Nqo1) , heme oxygenase 1 (gene: hmox1; protein: HO1) is among the nrf2 target genes. It catalyzes heme degradation with biliverdin, iron ions, and carbon monoxide (CO) as by-product, and HO-1 has been associated with ROS/RNS-driven oxidative stress responses [ 27 ].

Results from reverse transcription polymerase chain reaction and promoter assays reveal that HX-1171 increased the expression of NQO1 and HMOX1, encoding antioxidant-related enzymes, in A549 human lung epithelial cells.

May 13, 2019 · We investigated how altering iron in vivo affected hepatic expression of Bmp6, and downstream Bmp/Smad (Hamp1, Id1, Smad7) and Nrf2 (Nqo1, Hmox1, Gclc) target genes.

Polymorphisms in NRF2 and its target antioxidant genes: HMOX-1, NQO1, and MT, have been associated with cardiovascular diseases (CVDs) and diabetes in various ethnic groups, however, with variable results.

May 1, 2015 · The heme oxygenase 1 (HMOX1) and NADH:quinone oxidoreductase 1 (NQO1) genes are crucial mediators of cellular defence against oxidative stress. In the present study, we analysed the associations of HMOX1 (GT) n and NQO1 C609T polymorphisms with …

May 1, 2015 · The heme oxygenase 1 (HMOX1) and NADH:quinone oxidoreductase 1 (NQO1) genes are crucial mediators of cellular defence against oxidative stress. In the present study, we analysed the associations of HMOX1 (GT)n and NQO1 C609T polymorphisms with …

Nov 19, 2020 · Our results show that NQO1 overexpression leads to improvements in insulin sensitivity and in glucose, lipid and NAD + metabolism while providing protection from HFD-induced liver steatosis and...