May 18, 2023 · LoNA tethers nucleophosmin (NPM1), a GC-enriched protein, to fibrillin (FBL), a DFC-enriched protein, and drives the formation of the compartmentalized nucleolus via liquid–liquid phase...

May 14, 2024 · In our in vivo CRISPR RBP screen, fibrillarin (FBL) emerges as a crucial nucleolar protein that regulates AML cell survival, primarily through its phase separation domains rather than...

Aug 9, 2019 · Two key nucleolar proteins that have received the most attention are nucleophosmin (B23/NPM1) and fibrillarin (FBL). NPM1 is a highly expressed nucleolar protein that is particularly enriched in the granular component (GC), the outer layer of the nucleolus that is associated with assembling ribosomal proteins together with processed rRNA into ...

Sep 1, 2020 · Nucleolar proteins strongly implicated in driving LLPS include the dense fibrillar component constituent, fibrillarin (FBL), and the scaffold protein of the granular component, nucleophosmin...

May 18, 2023 · Here, we demonstrate that lncRNA LoNA tethers granular-component-enriched NPM1 to dense-fibrillar-component-enriched FBL and drives the formation of compartmentalized nucleolus via facilitating liquid-liquid phase separation of those two nucleolar proteins.

Nov 9, 2023 · Fibrillarin (Fbl) and Nucleophosmin (Npm1) are canonical domain markers for the DFC and the GC, respectively; both proteins have been shown to phase-separate in vitro and in vivo [6, 7].

Dec 5, 2019 · RPA194 is a Pol I component in FCs; Fibrillarin (FBL) is a key factor involved in 47S pre-rRNA processing in DFCs; and B23, also called NPM1, is associated with the later stage of pre-rRNA processing in GCs (Boisvert et al., 2007).

The FC is surrounded by the dense fibrillar component (DFC), which contains the ribonucleoprotein fibrillarin (FBL) (Fig. 1A and fig. S1, A and B). The GC phase is rich in negatively charged proteins such as nucleophosmin (NPM1) and nucleolin (23).

Jun 21, 2023 · In this study, we demonstrated that the nucleolar protein ZNF692 directly interacts with nucleophosmin 1 (NPM1). Knocking down ZNF692 resulted in the nucleolar redistribution of NPM1 in ring-like structures and reduced protein synthesis.

Aug 3, 2022 · In this study, we highlight a new role for nucleolar KLF16 in programming cap-independent translation by interacting with essential nucleolar proteins nucleophosmin1 (NPM1) and fibrillarin (FBL), and consequently, enhancing cellular stress tolerance and pathogenesis of …