NLRP3 was predominantly expressed in wobbler NeuN + cells (motor neuronal cells), as well as in Iba1 + (microglia) and GFAP + (astrocytes), which were in close proximity to motor neurons. Arrows indicate double-labeled cells.

The increased co-location of NLRP3 and ionized calcium binding adaptor molecule 1 (Iba1) protein expression supported that microglia in glial cells was the primary contributor for CUMS-induced PFC NLRP3 inflammasome activation in rats.

Oct 13, 2020 · Peripheral inflammation exacerbated the dysfunction of microglial clearance in a NLRP3-dependent manner. We quantified the Iba1+ microglia that were co-localized with Aβ 1-42 fragments to...

May 15, 2020 · In this study, we demonstrated that severe memory deficits occurred in the late phase of EAE, and cognitive deficits were ameliorated by treatment with MCC950, an inhibitor of the NLRP3...

Feb 3, 2025 · Here, we found that Aβ deposition in vivo directly triggered NLRP3 activation in APP/PS1 mice, which model many features of AD. Loss of NLRP3 increased glutamine- and glutamate-related metabolism and increased expression of microglial Slc1a3, which was associated with enhanced mitochondrial and metabolic activity.

May 21, 2018 · In the present study, we present evidences that microglial NLRP3 inflammasome activation is critical for dopaminergic neuronal loss and the subsequent motor deficits in the...

Nov 14, 2020 · In injured brains after tMCAO challenge, NLRP3 is upregulated in the penumbra and ischemic core regions, particularly in cells expressing Iba1 (a marker of macrophages). NLRP3 inflammasome activation including ASC speck formation, caspase-1 activation, and IL-1β maturation occurs in injured brains.

Sep 1, 2024 · Western blot assay examined the protein levels of IBA1, CD68, TXNIP, NLRP3, ASC, and Cleaved Caspase-1 in BV-2 cells. The contents of inflammatory factor were detected by ELISA assay. The co-immunoprecipitation assay examined the …

Jan 25, 2023 · To study the relationship between neural plasticity damage caused by this paradigm and NLRP3, we used microglial cell-specific protein antibody Iba1 to specifically label microglial cells for IHC tests, and the ImageJ visualization software to analyze the activation of …

Immunofluorescence staining showed an up-regulation in NLR Family Pyrin Domain Containing 3 (NLRP3) immunoreactivity in Iba1-labled microglia together with total Iba1 and NLRP3 expression in the brain following tMCAO, while down-regulated by CEP treatment.