Jul 15, 2000 · We have engineered a self-inactivating retroviral vector containing multiple (3 or 6) nuclear factor of activated T-cell (NFAT)-binding sites, followed by the minimal IL2 promoter and the reporter gene GFP. Jurkat cells, primary T-cell blasts, and T-cell clones were transduced with high efficiency (20%-40%).

Recombinant Jurkat T-cells expressing an enhanced GFP (eGFP) gene under the control of NFAT response elements located upstream of the minimal TATA promoter. Activation of the NFAT signaling pathway can be monitored by examining eGFP expression.

Jun 1, 2005 · In this Review, I provide an overview of recent advances in our understanding of the regulation, mechanism of action and functions of NFAT proteins in cells of the immune system, with a focus on...

In this study, we generated a novel platform cell line, CD4-2D3, in which GFP reporter was expressed by NFAT activation via TCR signaling, for correctly evaluating functional avidity of HLA class II-restricted TCRs.

Jan 28, 2019 · To detect the signal induced by SABRs, we used NFAT-GFP-Jurkat cells, which express GFP after receiving a signal via CD3ζ.

Jul 15, 2000 · We have engineered a self-inactivating retroviral vector containing multiple (3 or 6) nuclear factor of activated T-cell (NFAT)-binding sites, followed by the minimal IL2 promoter and the reporter gene GFP.

Mar 20, 2023 · In this study, we generated a novel platform cell line, CD4-2D3, in which GFP reporter was expressed by NFAT activation via TCR signaling, for correctly evaluating functional avidity of HLA class II-restricted TCRs.

Recombinant Jurkat T cells expressing both firefly luciferase and enhanced GFP (eGFP) under the control of NFAT response elements located upstream of the minimal TATA promoter. Activation of the NFAT signaling pathway can be monitored by examining either firefly luciferase or eGFP expression. Background

NFAT-binding sites, followed by the minimal IL2 promoter (IL-2Pmin) and the reporter gene, encoding the enhanced green fluorescent protein (GFP from Clontech Laboratories, Palo Alto, CA).

To examine the dynamics and efficiency of NFAT activation in Ag-experienced T cells upon encounter with APC that deliver a strong TCR as well as co-stimulatory signals in vivo, we seeded mice with NFAT-GFP-expressing HA-T CM, prepared inflamed, tumor-draining popliteal LNs for multiphoton intravital microscopy (MP-IVM) analysis (Mempel et al ...