Jan 2, 2025 · By using pharmacological and optogenetic manipulations in vitro and in vivo, we found that NE reduces the excitability of aPC pyramidal neurons directly via α2 receptors and that it...

Jan 8, 2025 · Our findings identify locus coeruleus (LC)-induced NE oscillations as the key driver of slow vasomotion, which in turn facilitates glymphatic clearance during natural sleep.

Dec 15, 2010 · Postsynaptic depolarization induced by ChR2 can be combined with two-photon glutamate uncaging to potentiate visually identified dendritic spines. ChR2-mediated stimulation of presynaptic axons induces neurotransmitter release and reliably activates postsynaptic spines.

Jul 3, 2024 · Here, we report that responses of norepinephrine (NE) neurons in the locus coeruleus (LC) of non-human primates to behaviorally relevant sensory stimuli promote visual discrimination in a spatially selective way. LC-NE neurons spike in response to a visual stimulus appearing in the contralateral hemifield only when that stimulus is attended.

Aug 5, 2015 · Here we propose a separate neuromodulatory system underlying immediate, acute stress-induced anxiety. The locus coeruleus-noradrenergic (LC-NE) system is a small, tightly packed pontine brain region that sends numerous projections to the forebrain and spinal cord.

Jun 9, 2022 · We first tested whether the timely release of norepinephrine (NE) or serotonin (5HT) after visual stimulation potentiates or depresses visual responses in V1 in a manner consistent with the...

Channelrhodopsin-2 (ChR2) is a light-gated ion channel, which is native to the eyespot of the unicellular alga Chlamydomonas reinhardtii (1). ChR2 is involved in the photoperception of the alga, leading to its phototactic behavior. ChR2 represents …

Nov 2, 2015 · To optically activate populations of LC-NE neurons, lentivirus expressing channelrhodopsin2 (ChR2) under the control of a catecholaminergic-specific promoter was injected into the LC of rats.

Optogenetic stimulation of neurons using Channelrhodopsin-2 (ChR2) has become a widely used tool in neuroscience. ChR2, a directly light-gated cation channel of the green algae Chlamydomonas reinhardtii, allows depolarizing and firing neurons with …

Oct 31, 2013 · Here we report optical inhibition of motor neuron and muscle activity in vivo in the cooled sciatic nerves of Thy1-ChR2-EYFP mice. We also demonstrate all-optical single-wavelength control of neuronal excitation and inhibition without co-expression of inhibitory and excitatory opsins.