Sep 26, 2023 · Myrlin interacts with the MLL1 complex. Myrlin CRISPRi compromised MLL1 occupancy in the Myb locus, decreasing CDK9 and RNA Pol II binding and resulting in Pol II pausing in the Myb first exon/intron. Thus, Myrlin directly participates in activating Myb transcription by recruiting MLL1.

Nov 16, 2007 · Again, using an immunoprecipitation strategy we found that c-Myb is associated with MLL1, WDR5, RbBp5, and Ash2L, which are previously identified members of a multi-protein MLL-SET1 histone H3 lysine (K) 4 methyltransferase complex.

NMR structure of KIX-c-Myb-MLL1 ternary complex (PDB: 2AGH) and compounds inhibiting the MLL1-CBP interaction. a Overview of MLL1 peptide binding to KIX-c-Myb complex. MLL1 is shown as sticks with C atom in yellow. KIX and c-Myb are shown as surfaces in cyan and magenta, respectively.

Nov 20, 2009 · We demonstrated in vitro with purified proteins and an H3 peptide, that c-Myb contributed to the HMT activity of the MLL1 complex. In leukemia patients being treated with a c-myb targeted antisense oligodeoxynucleotide (ASODN), and in leukemic cell lines, silencing c-myb evoked a significant decrease in H3K4 methylation demonstrating biological ...

This work clearly shows that Myrlin eRNA directly participates in activating Myb transcription via KMT2A/MLL1 recruitment in MEL cells. We showed that Myrlin may help to recruit or stabilize CDK9 at Myb through KMT2A, but other molecules could also contribute and should be …

MLL1, located on human chromosome 11, is disrupted in distinct recurrent chromosomal translocations in several leukemia subsets. Studying the MLL1 gene and its oncogenic variants has provided a paradigm for understanding cancer initiation and maintenance through aberrant epigenetic gene regulation.

Sep 26, 2023 · Myrlin CRISPRi compromised MLL1 occupancy in the Myb locus, decreasing CDK9 and RNA Pol II binding and resulting in Pol II pausing in the Myb first exon/intron. Thus, Myrlin directly participates in activating Myb transcription by recruiting MLL1.

Oct 12, 2023 · Myrlin and Myb are coordinately regulated during erythroid differentiation. Myrlin TSS deletion using CRISPR/Cas9 reduced Myrlin and Myb expression and LDB1 complex occupancy at the Myb enhancers, compromising enhancer contacts and reducing RNA Pol II occupancy in the locus.

Nov 16, 2007 · We hypothesize that in the case of MLL1, this interaction contributes to leukemic transformation of hematopoietic cells, and may more broadly contribute to malignant transformation in those tumors in which c-Myb has been reported to …

Nov 20, 2009 · C-Myb Associates with MLL1 through Menin, Augments MLL1's H3K4 Methylation Activity, and Regulates Hoxa9 and Meis1 Gene Expression in Primary Human Leukemia Cells.