Nov 1, 2006 · Demonstration of acid-fast bacilli (AFB) in a smear made from a clinical specimen provides a preliminary diagnosis of mycobacterial disease, while the isolation of mycobacteria on culture provides a definite diagnosis of tuberculosis or disease due to mycobacteria other than M. tuberculosis (MOTT bacilli) or non-tuberculous mycobacteria (NTM).

The BD BACTEC™ MGIT™ 960 system is for high-volume mycobacteria growth, detection and susceptibility testing and is designed to meet the needs of medium- and high-volume laboratories.

• Studies have shown that, using MGIT, respiratory specimens that are culture negative at 4 weeks have a 95–100% probability of a final negative result at 6 weeks • Consider reporting interim report (e.g., No growth of mycobacteria at 4 weeks. Final report to follow)

The AFB culture is the inoculation of a clinical specimen onto culture media Becton-Dickinson Mycobacteria Growth Indicator Tube (B-D MGIT broth) and Lowenstein-Jensen (L-J) media slant, incubation at 37°C for up to six (6) weeks and detection of growth or no growth during this incubation period.

• Most specimens for AFB testing come from non-sterile sites – Despite decontamination, some contamination of culture media is to be expected – Common contaminants include molds, yeast, bacteria, and some NTM • Acceptable contamination rate for liquid media is …

Mycobacteria Growth Indicator Tube (MGIT) is intended for the culture, detection and recovery of mycobacteria. The MGIT Mycobacteria Growth Indicator Tube contains 7 mL of modified Middlebrook 7H9 Broth base.

ARUP’s AFB lab performs testing seven days a week isolating, identifying, and performing susceptibility on most mycobacteria species. We use a combination of MALDI-TOF, PCR, and 16S rRNA sequencing to obtain species-level identification using …

» AFB smear microscopy for patients suspected of pulmonary TB disease » Liquid and solid culture for identification and antimicrobial susceptibility testing » Rapid nucleic acid amplification tests on initial respiratory specimens

Liquid media are generally poured into test tubes or culture bottles. The OSPHL uses MGIT liquid media to grow and isolate AFB organisms. AFB are first detected by fluorescence microscopy. Identification of AFB as either MTB or M. avium occurs by …

The BACTEC Mycobacteria Growth Indicator Tube 960 (MGIT 960) system was applied for recovery of mycobacteria from extrapulmonary specimens and compared with solid media (Löwenstein-Jensen and Stonebrink).