Plasmid pLNCX myr Akt1 from Dr. Joan Brugge's lab contains the insert Akt1 and is published in Proc Natl Acad Sci U S A. 2007 Mar 6. 104 (10):3787-92. This plasmid is available through Addgene.

Jan 16, 2009 · As an experimental model, we use embryonic fibroblasts from knock-out mice without major ATP-ADP exchange enzymes, in which we (re)introduce the ATP/ADP exchange enzyme adenylate kinase-1 (AK1) and deliberately manipulate its spatial positioning by coupling to different artificial location tags.

USERS CAN NOW UPLOAD LARGE FILES AT AK1 DROPBOX! Post any Mugen Characters you would like to share or chat about. If the character is uploaded in the Downloads section page, just remember that it will also automatically create a topic in this forum due to any updates from the Characters section.

To construct Myr-HA-Akt1, the c-Src myristoylation sequence was fused in frame to the N terminus of the HA-Akt (wild-type) coding sequence. Myr-HA-Akt1 is a constitutively active mutant of Akt1, and it is also referred as Akt (ca).

Conclusion: Akt1-myocardin-SRF signaling induces myofibroblast differentiation. Significance: The role of Akt1 on myofibroblast differentiation highlights it as a potential target for the treatment of fibrotic diseases.

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

Jun 10, 2022 · The cavities, which were complementary to the protein secondary structure, acted as a peptide conformational imprint (PCI) for adenylate kinase 1 (AK1). We established a promising strategy to examine the binding affinities of human AK1 in conformational dynamics using the peptide-imprinting method.

The Myr EC 350 is a modular paraffin embedding center featuring innovative design as well as easy operation. Multiple configurations of instrument set-up offer a simple and flexible adaptation to the working methods in the respective lab.

By transfection-complementation of AK1 variants and comparison with yellow fluorescent protein controls, we found that motility and spreading were enhanced in cells with AK1 with a focal contact guidance tag. Intermediary enhancement was observed in cells with membrane-targeted or cytosolic AK1.

Oct 26, 2007 · Adenosine treatment bypassed AK1 deficiency and restored post-ischemic flow to wild-type levels, achieving phenotype rescue. AK1 phosphotransfer thus transduces stress signals into adequate vascular response, providing linkage between cell bioenergetics and coronary flow.