Both coding exons of the c-myc gene contribute to its posttranscriptional regulation in the quiescent liver and regenerating liver and after protein synthesis inhibition.

Based on whole genome sequencing (WGS) data we could show that part of the MYC gene, which carries the coding exons 2 and 3, is gained and inserted IGHA1 the IGH locus within the switch region of IGHA1 (IGHA1 switch region chr14:106,715,033-106,178,629bp, hg19, Hübschmann et al., unpublished).

We demonstrated that the presence of one of the two coding exons, exon 2 or exon 3, is sufficient to confer a level of expression of transgene mRNA similar to that of endogenous c-myc in various adult tissues as well as after partial hepatectomy or after protein synthesis inhibition.

Aug 1, 2017 · CMYC is composed of three exons spanning over 4 kb with the second and third exons encoding most MYC protein. These two exons have at least 70% sequence homology among species. However, exon1 is untranslated whose sequence is not as well conserved through evolution.

Instead, coding elements in myc exons 2 and 3 target c- myc mRNA for down-regulation during differentiation. In studies presented here, we characterize the mechanism by which c- myc expression is posttranscriptionally down-regulated during C2C12 myogenic differentiation.

To identify cis-acting elements that target c-myc mRNA for downregulation during myogenesis, we stably transfected C2C12 cells with mutant myc genes or chimeric genes in which various myc sequences were fused to the human beta-globin gene or to the bacterial chloramphenicol acetyltransferase (CAT) gene.

Coding sequences from MYC exons 2 and 3 are necessary for regulated cycloheximide inducibility (CHX Ind) of myc mRNA during myoblast differentiation. Induction in myc mRNA levels was examined...

Jun 19, 1998 · Murine C2C12 myoblasts induced to differentiate into multinucleated myotubes decrease their levels of c-myc mRNA 3-10-fold through posttranscriptional mechanisms that recognize regulatory elements contained in protein-coding sequences in exons 2 …

Mar 3, 2020 · We show that an ultraconserved, NMD-determinant exon in the RNA-binding protein SRSF3 is particularly responsive to Myc signaling. Our results implicate Myc signaling as a regulator of alternative splicing-coupled NMD (AS-NMD) as part of a program of growth control.

The human c‐myc gene consists of three exons transcribed from two distinct promoters and the function of the first, noncoding exon is unknown. In COLO 320 cells, there co‐exist normal and truncated (i.e., lacking exon 1) c‐myc genes, both of which are transcribed.