MDM2 amplification represents the leading oncogenic pathway and diagnostic hallmark of liposarcoma, whose assessment is based on Fluorescence In Situ Hybridization (FISH) analysis.

The MDM2 gene and its neighboring gene CDK4 are amplified, which can be detected by molecular methods such as reverse transcription–polymerase chain reaction (RT-PCR) and FISH. The resultant MDM2 and CDK4 protein overexpression can be detected by IHC.

In this study, we found that MDM2 or CDK4 protein expression by IHC or MDM2 gene amplification by FISH were occasionally present in head and neck osteosarcomas, while all benign fibro-osseous lesions were negative for MDM2 and …

Fluorescent in situ hybridization (FISH) for amplification of MDM2 has been considered the gold standard for diagnosis in these situations. Immunostaining for MDM2 and/or CDK4 has been adopted as a surrogate method because of its high concordance rate with FISH and lower cost.

Atypical lipomatous tumor and dedifferentiated liposarcoma. Clearly indicate clinical reason for testing provide the following information: differential diagnosis, site of specimen, and clinical information. Option III: Full surgical pathology consultation (can be ordered with option II.)

Jan 22, 2025 · MDM2 IHC is used to detect overexpression of MDM2. A formalin-fixed, paraffin-embedded (FFPE) tissue block is preferred specimen type or one (1) unbaked, unstained slide for H&E staining (required) and two to three (2-3) positively charged unstained slides (all cut at 4-5 microns) for each test/antibody ordered.

To analyze the utility and limitations of ancillary techniques, we studied 11 cases of DDLPS in challenging conditions and 17 cases of nonlipogenic high-grade sarcomas with immunohistochemistry (IHC) for p16, CDK4, and MDM2 and automated dual-color in situ hybridization (DISH) for MDM2 amplification.

MDM2 (BSB-64) is a mouse monoclonal antibody (MMab) for immunohistochemistry from Bio SB. Used to distinguish dedifferentiated liposarcoma (positive) from poorly differentiated sarcomas (negative), as well as low grade osteosarcoma (positive) from benign fibrous and fibro-osseous lesions (negative).

Detection of MDM2/CDK4 protein overexpression by IHC can be used by pathologists to diagnose WDLPS and DDLPS. Considering a strong and diffuse immunostaining pattern in most of the neoplastic cells achieves the best results in identifying these tumours.

Murine Double Minute Clone 2, located at 12q15, is an oncogene that codes for an oncoprotein of which the association with p53 was discovered 30 years ago. The most important function of MDM2 is to control p53 activity; it is in fact the best documented negative regulator of p53.