Oct 17, 2023 · In this study, we employed spatial transcriptomics (ST) to examine ketamine’s lasting effects in the medial prefrontal cortex (mPFC) and hippocampus of mice subjected to chronic social defeat...

Mar 17, 2022 · Here, we uncover a non-canonical function of OLIG2 in transcriptional repression to modulate myelinogenesis by functionally interacting with tri-methyltransferase SETDB1.

Magnified images of the white matter of Olig2 plp-iko mice (C ′) and wt mice (B ′) immunostained for MBP (red), CC1 (red), and Olig2 (white). Decreased colocalization of Olig2 and CC1 (B ″, C ″) displays effective deletion of Olig2 specifically in differentiated oligodendrocytes.

The lancelet “Mbp” gene lacks the OLIG1/2 and SOX10 binding sites that characterize vertebrate Mbp homologs, raising the possibility that insertion of cis-regulatory elements might have been involved in evolution of the myelinating programme.

In this study, myelin basic protein-activated T (MBP-T) cells were passively immunized to improve the SCI microenvironment. Olig2-overexpressing NSCs were infected with a lentivirus carrying the enhanced green fluorescent protein (GFP) reporter gene to generate Olig2-GFP-NSCs that were transplanted into the injured site to differentiate into OLs.

Mar 10, 2011 · We present evidence that OLIG2 controls the switch by reversible phosphorylation on Serine 147 (S147), a predicted protein kinase A (PKA) target; phosphorylation at this site is required for patterning of the ventral neuroepithelium and MN specification, whereas dephosphorylation favors OLP specification.

May 7, 2023 · OLIG2, MYT1L, and their synergistic (OLIG2 + MYT1L) transfected groups showed an intense expression of MYT1L, OLIG2, NG2, and MBP proteins after 7 days of incubation compared to the control group in the oligo induction medium (Figure 6 C).

Disrupting OLIG2-SETDB1 complex in the early OL lineage causes hypomyelination in mice. Our findings disclose a distinct mechanism that OLIG2 composed a TF-Epigenetic complex to conduct gene inhibition, for OL cell fate determination and myelination.

Nov 9, 2022 · Analysis of genomic occupancy in immature and mature OLs revealed that Olig2 targets the enhancers of key myelination-related genes for OL maturation from immature OLs.

Jan 13, 2022 · This method enables generation of A2B5+ (an early marker for OPCs) iOPCs within 2 weeks retaining the ability to differentiate into MBP-positive mature oligodendrocytes.