Hou et al. show that cardiac fibroblasts facilitate infiltrating Ly6Chi and Ly6Clo monocytes to become macrophages. IL-17A trans-signaling through cardiac fibroblasts increases MerTK shedding and promotes a pro-inflammatory and pro-tissue remodeling gene expression profile in Ly6Chi monocyte-derived macrophages.

To test the overall contribution of the Cd36-Mertk axis, MI was induced in Cd36 −/− Mertk −/− double-knockout mice and led to increases in myocardial rupture. These data implicate monocyte CD36 in the mitigation of early infarct size and transition to …

Infarction triggers the early recruitment of Ly6CHI monocytes, which transition into resolving Ly6CLO macrophages. Previous studies have linked key apoptotic cell receptors, such as MerTK on cardiac Ly6cLO macrophages, to be required for clearance of dying cardiomyocytes.

Sep 13, 2021 · Transplantation of BM-MSCs markedly promoted a phenotypic switch from pro-fibrotic Ly6Chi subset to restorative Ly6Clo subpopulation by secreting paracrine cytokines IL-4 and IL-10 from the...

Here, we show that infiltrating Ly6C hi monocytes, their macrophage descendants, and neutrophils spatially and temporally overlap in the centrilobular necrotic areas during the necroinflammatory and resolution phases of AILI.

Jan 22, 2014 · The role of MerTK in shaping the monocyte/macrophage response after PVA sponge implantation was investigated using mice that are deficient in MerTK signaling (MerTK −/−). The day 14 wound was chosen for analysis because the highest level of MerTK expression was detected on Ly6C low wound macrophages at this time point ( Figure 1C ).

The scavenger receptor cluster of differentiation (CD)36 protein was detected on cardiac Ly6cHI monocytes, and bone marrow-derived Cd36 was essential for both early phagocytosis of dying cardiomyocytes and for smaller infarct sizes in female and …

To define macrophages we took advantage of recent findings that co-expression of the tyrosine kinase Mer (MerTK) and CD64 provides a robust marker for macrophages in the liver . F4/80 was not a sufficient marker of macrophages in this setting, since it also marked eosinophils and monocytes (Fig. 1A, and ).

Sep 13, 2021 · The activation of the MerTK-ERK pathway was accompanied with the secretion of MMP12. The correlation among MerTK, ERK1/2, and MMP12 was evidenced by utilizing a MerTK inhibitor in Ly6C lo macrophages under stimulation with apoptotic bodies.

Nov 15, 2024 · In this study, we examined surface integrins' role in TAM recruitment and phenotypic programming in a 4T1-induced murine breast tumor model. Our findings show that integrin α5β1 is upregulated in CD11b+Ly6Chi monocytes in the bone marrow and blood by day 10 after tumor induction.